Production of human pluripotent stem cell therapeutics under defined xeno-free conditions: progress and challenges.
Production of human pluripotent stem cell therapeutics under defined xeno-free conditions: progress and challenges.
复制标题
在定义的无XENO条件下生产人类多能干细胞疗法：进展和挑战。
DOI：
10.1007/s12015-014-9544-x
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发表时间：
2015-02
影响因子：
4.8
通讯作者：
Tzanakakis, Emmanuel S.
中科院分区：
文献类型：
--
作者：
Fan, Yongjia;Wu, Jincheng;Ashok, Preeti;Hsiung, Michael;Tzanakakis, Emmanuel S.
关键词：
人类多能干细胞（hPSC），包括人类胚胎干细胞（hESC）和诱导多能干细胞（hiPSC）的最新进展使我们更接近实现其临床潜力。尽管如此，组织工程和再生医学应用将需要产生远远超出实验室规模的hPSC产品。这也要求在完全限定的、无异种系统中并且以可再现的方式生产hPSC治疗剂。为了实现这一目标，我们总结了目前的发展，在确定的媒体不含动物源性成分的hPSC文化。生物启发和合成的细胞外基质的附着生长和分化的hPSC也进行了审查。鉴于在无异源培养基和底物开发中的大多数进展已经在二维而不是三维培养系统中被证明，从前者到后者的翻译造成了独特的困难。这些挑战的背景下讨论的hPSC作为聚集体的培养平台，在微载体上或封装后的生物相容性支架。
Recent advances on human pluripotent stem cells (hPSCs), including human embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs) have brought us closer to the realization of their clinical potential. Nonetheless, tissue engineering and regenerative medicine applications will require the generation of hPSC products well beyond the laboratory scale. This also mandates the production of hPSC therapeutics in fully-defined, xeno-free systems and in a reproducible manner. Toward this goal, we summarize current developments in defined media free of animal-derived components for hPSC culture. Bioinspired and synthetic extracellular matrices for the attachment growth and differentiation of hPSCs are also reviewed. Given that most progress in xeno-free medium and substrate development has been demonstrated in two-dimensional rather than three dimensional culture systems, translation from the former to the latter poses unique difficulties. These challenges are discussed in the context of cultivation platforms of hPSCs as aggregates, on microcarriers or after encapsulation in biocompatible scaffolds.
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