Genome-wide mapping of Arabidopsis thaliana origins of DNA replication and their associated epigenetic marks.
Genome-wide mapping of Arabidopsis thaliana origins of DNA replication and their associated epigenetic marks.
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DOI：
10.1038/nsmb.1988
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发表时间：
2011-03
影响因子：
16.8
通讯作者：
中科院分区：
文献类型：
--
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基因组的完整性需要染色体的忠实复制。复制起点，即DNA复制起始的基因组位点，分散在整个基因组中。在这里，我们通过对新合成的DNA进行高通量测序来分析拟南芥的起源，并在全基因组范围内确定了约1500个推定的起源。这得到了ChIP芯片实验的支持，以鉴定ORC 1和CDC 6结合位点。通过测量新生DNA链的丰度独立地验证起源活性。大多数拟南芥起源区域的中点优先位于基因的5'半内，稍微富集G+C、组蛋白H2A.Z、H3 K4 me 2/3和H4 K5 ac，并且耗尽H3 K4 me 1和H3 K9 me 2。我们的数据建立的基础，了解拟南芥中的DNA复制起点的表观遗传规范，并对其他真核生物的影响。
Genomic integrity requires faithful chromosome duplication. Origins of replication, the genomic sites where DNA replication initiate, are scattered throughout the genome Their mapping at a genomic scale in multicellular organisms has been challenging. Here we have profiled origins in Arabidopsis by high-throughput sequencing of newly-synthesized DNA and identified ~1500 putative origins genome-wide. This was supported by ChIP-chip experiments to identify ORC1 and CDC6 binding sites. Origin activity was validated independently by measuring the abundance of nascent DNA strands. The midpoints of most Arabidopsis origin regions are preferentially located within the 5’ half of genes, slightly enriched in G+C, histone H2A.Z, H3K4me2/3 and H4K5ac, and depleted of H3K4me1 and H3K9me2. Our data establish the basis for understanding the epigenetic specification of DNA replication origins in Arabidopsis and have implications for other eukaryotes.
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