Cancer-associated fibroblasts are positively correlated with metastatic potential of human gastric cancers.
Cancer-associated fibroblasts are positively correlated with metastatic potential of human gastric cancers.
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DOI：
10.1186/1756-9966-29-66
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发表时间：
2010-06-08
期刊：
影响因子：
--
通讯作者：
Bi J
中科院分区：
文献类型：
--
作者：
Zhi K;Shen X;Zhang H;Bi J
胃癌患者的预后是很难预测的，因为在建立的组织病理学特征的缺陷。癌相关成纤维细胞（CAFs）在促进肿瘤生长、侵袭和转移中起重要作用。提示胃癌患者CAFs的发生程度可能有助于判断胃癌患者的预后。通过免疫组化和实时荧光定量PCR实验，比较肿瘤和正常组织标本中CAFs特异性标志物或CAFs分泌蛋白的表达。根据免疫组化染色结果对CAFs的患病程度进行分级，并进一步分析CAFs的患病率与其他可能影响胃癌患者预后的肿瘤特征之间的相关性。近80%的正常胃组织CAFs染色呈阴性或弱阳性，而60%以上的胃癌组织CAFs染色呈中阳性或强阳性。Realtime-PCR结果显示，FAP、SDF-1和TGF-β1在胃癌组织中的表达明显高于正常胃组织。进一步分析发现CAFs的患病率与肿瘤大小、肿瘤深度、淋巴结转移、肝转移或腹膜转移有关。反应性癌相关成纤维细胞（reactive cancer associated fibroblasts，CAFs）在胃癌组织中大量存在，且CAFs的检出率与肿瘤大小、肿瘤深度及肿瘤转移有关，为判断胃癌患者的预后提供了一定的依据。
The prognosis of gastric cancer patients is difficult to predict because of defects in establishing the surgical-pathological features. Cancer-associated fibroblasts (CAFs) have been found to play prominent role in promoting tumor growth, invasion and metastasis. Thus raises the hypothesis that the extent of CAFs prevalence may help to establish the prognosis of gastric cancer patients. Immunochemistry and realtime-PCR experiments were carried out to compare the expression of proteins which are specific markers of CAFs or secreted by CAFs in the tumor and normal tissue specimens. The extent of CAFs' prevalence was graded according to immunochemical staining, and correlation was further analyzed between CAFs' prevalence and other tumor characteristics which may influence the prognosis of gastric cancer patients. Nearly 80 percent of normal gastric tissues were negative or weak positive for CAFs staining, while more than 60 percent of gastric cancer tissues were moderate or strong positive for CAFs staining. Realtime-PCR results also showed significant elevated expression of FAP, SDF-1 and TGF-β1 in gastric cancer tissues compared to normal gastric tissues. Further analysis showed that CAFs' prevalence was correlated with tumor size, depth of the tumor, lymph node metastasis, liver metastasis or peritoneum metastasis. Reactive cancer associated fibroblasts (CAFs) were frequently accumulated in gastric cancer tissues, and the prevalence of CAFs was correlated with tumor size, depth of the tumor and tumor metastasis, thus give some supports for establishing the prognosis of the gastric cancer patients.
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影响因子：
11.2
作者：
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通讯作者：
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DOI：
10.1111/j.1440-1746.2007.04991.x
发表时间：
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影响因子：
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作者：
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