A single-cell transcriptomic landscape of the lungs of patients with COVID-19.
A single-cell transcriptomic landscape of the lungs of patients with COVID-19.
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COVID-19 患者肺部的单细胞转录组图景
DOI：
10.1038/s41556-021-00796-6
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发表时间：
2021-12
影响因子：
21.3
通讯作者：
Bian XW
中科院分区：
文献类型：
--
作者：
Wang S;Yao X;Ma S;Ping Y;Fan Y;Sun S;He Z;Shi Y;Sun L;Xiao S;Song M;Cai J;Li J;Tang R;Zhao L;Wang C;Wang Q;Zhao L;Hu H;Liu X;Sun G;Chen L;Pan G;Chen H;Li Q;Zhang P;Xu Y;Feng H;Zhao GG;Wen T;Yang Y;Huang X;Li W;Liu Z;Wang H;Wu H;Hu B;Ren Y;Zhou Q;Qu J;Zhang W;Liu GH;Bian XW
肺是严重急性呼吸综合征冠状病毒2（SARS-CoV-2）的主要靶器官，使呼吸衰竭成为2019年冠状病毒病（COVID-19）相关死亡率的主要原因。然而，我们对SARS-CoV-2感染如何驱动肺部病理的细胞和分子理解是有限的。在这里，我们构建了COVID-19患者肺部的多组学和单核转录组图谱，其中整合了组织学，转录组学和蛋白质组学分析。我们的工作揭示了与SARS-CoV-2感染相关的不同肺和浸润免疫细胞群的病理标志的分子基础。我们报告了炎症过度、肺泡上皮细胞衰竭、血管变化和纤维化的分子指纹，并将肺实质衰老确定为COVID-19病理学的分子状态。此外，我们的数据表明，FOXO 3A抑制是与COVID-19肺纤维化相关的成纤维细胞向肌成纤维细胞转化的潜在机制。我们的工作描绘了COVID-19患者肺部的全面细胞和分子图谱，并提供了对SARS-CoV-2相关肺损伤的见解，促进了生物标志物的鉴定和对症治疗的开发。
The lung is the primary organ targeted by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), making respiratory failure a leading coronavirus disease 2019 (COVID-19)-related mortality. However, our cellular and molecular understanding of how SARS-CoV-2 infection drives lung pathology is limited. Here we constructed multi-omics and single-nucleus transcriptomic atlases of the lungs of patients with COVID-19, which integrate histological, transcriptomic and proteomic analyses. Our work reveals the molecular basis of pathological hallmarks associated with SARS-CoV-2 infection in different lung and infiltrating immune cell populations. We report molecular fingerprints of hyperinflammation, alveolar epithelial cell exhaustion, vascular changes and fibrosis, and identify parenchymal lung senescence as a molecular state of COVID-19 pathology. Moreover, our data suggest that FOXO3A suppression is a potential mechanism underlying the fibroblast-to-myofibroblast transition associated with COVID-19 pulmonary fibrosis. Our work depicts a comprehensive cellular and molecular atlas of the lungs of patients with COVID-19 and provides insights into SARS-CoV-2-related pulmonary injury, facilitating the identification of biomarkers and development of symptomatic treatments.
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影响因子：
64.8
作者：
Gillich A;Zhang F;Farmer CG;Travaglini KJ;Tan SY;Gu M;Zhou B;Feinstein JA;Krasnow MA;Metzger RJ
通讯作者：
Metzger RJ
DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Anders S;Pyl PT;Huber W
通讯作者：
Huber W
影响因子：
14.8
作者：
Krishnaswami SR;Grindberg RV;Novotny M;Venepally P;Lacar B;Bhutani K;Linker SB;Pham S;Erwin JA;Miller JA;Hodge R;McCarthy JK;Kelder M;McCorrison J;Aevermann BD;Fuertes FD;Scheuermann RH;Lee J;Lein ES;Schork N;McConnell MJ;Gage FH;Lasken RS
通讯作者：
Lasken RS
影响因子：
64.8
作者：
Delorey TM;Ziegler CGK;Heimberg G;Normand R;Yang Y;Segerstolpe Å;Abbondanza D;Fleming SJ;Subramanian A;Montoro DT;Jagadeesh KA;Dey KK;Sen P;Slyper M;Pita-Juárez YH;Phillips D;Biermann J;Bloom-Ackermann Z;Barkas N;Ganna A;Gomez J;Melms JC;Katsyv I;Normandin E;Naderi P;Popov YV;Raju SS;Niezen S;Tsai LT;Siddle KJ;Sud M;Tran VM;Vellarikkal SK;Wang Y;Amir-Zilberstein L;Atri DS;Beechem J;Brook OR;Chen J;Divakar P;Dorceus P;Engreitz JM;Essene A;Fitzgerald DM;Fropf R;Gazal S;Gould J;Grzyb J;Harvey T;Hecht J;Hether T;Jané-Valbuena J;Leney-Greene M;Ma H;McCabe C;McLoughlin DE;Miller EM;Muus C;Niemi M;Padera R;Pan L;Pant D;Pe'er C;Pfiffner-Borges J;Pinto CJ;Plaisted J;Reeves J;Ross M;Rudy M;Rueckert EH;Siciliano M;Sturm A;Todres E;Waghray A;Warren S;Zhang S;Zollinger DR;Cosimi L;Gupta RM;Hacohen N;Hibshoosh H;Hide W;Price AL;Rajagopal J;Tata PR;Riedel S;Szabo G;Tickle TL;Ellinor PT;Hung D;Sabeti PC;Novak R;Rogers R;Ingber DE;Jiang ZG;Juric D;Babadi M;Farhi SL;Izar B;Stone JR;Vlachos IS;Solomon IH;Ashenberg O;Porter CBM;Li B;Shalek AK;Villani AC;Rozenblatt-Rosen O;Regev A
通讯作者：
Regev A
影响因子：
158.5
作者：
Ackermann, Maximilian;Verleden, Stijn E.;Jonigk, Danny
通讯作者：
Jonigk, Danny