Porphyromonas gingivalis Components/Secretions Synergistically Enhance Pneumonia Caused by Streptococcus pneumoniae in Mice.
Porphyromonas gingivalis Components/Secretions Synergistically Enhance Pneumonia Caused by Streptococcus pneumoniae in Mice.
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DOI：
10.3390/ijms222312704
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发表时间：
2021-11-24
影响因子：
5.6
通讯作者：
Mitani A
中科院分区：
文献类型：
--
作者：
Okabe T;Kamiya Y;Kikuchi T;Goto H;Umemura M;Suzuki Y;Sugita Y;Naiki Y;Hasegawa Y;Hayashi JI;Kawamura S;Sawada N;Takayanagi Y;Fujimura T;Higuchi N;Mitani A
肺炎链球菌是呼吸道感染的重要病原体。虽然牙周细菌已被证明影响呼吸道感染，如吸入性肺炎，S。肺炎链球菌和牙龈卟啉单胞菌（一种牙周病细菌）对肺炎球菌感染的影响尚不清楚。为了研究牙龈卟啉单胞菌是否加速肺炎球菌感染，我们测试了牙龈卟啉单胞菌培养上清液（PgSup）对肺炎球菌感染的影响。肺炎感染的小鼠。小鼠气管内注射S.分析了肺炎链球菌和PgSup诱导肺炎的情况，并分析了肺组织病理学切片以及肺中中性粒细胞和巨噬细胞的绝对数量和频率。通过qPCR和ELISA检查促炎细胞因子/趋化因子表达。S.肺炎链球菌感染小鼠和S.肺炎链球菌和PgSup混合感染小鼠，混合感染小鼠肺部炎症更明显。单核/巨噬细胞和中性粒细胞的比例在S.肺炎感染的小鼠和混合感染的小鼠。与S.肺炎单独感染。我们证明PgSup增强了由S.这表明牙龈卟啉单胞菌产生的毒力因子参与呼吸道感染如吸入性肺炎的恶化。
Streptococcus pneumoniae is an important causative organism of respiratory tract infections. Although periodontal bacteria have been shown to influence respiratory infections such as aspiration pneumonia, the synergistic effect of S. pneumoniae and Porphyromonas gingivalis, a periodontopathic bacterium, on pneumococcal infections is unclear. To investigate whether P. gingivalis accelerates pneumococcal infections, we tested the effects of inoculating P. gingivalis culture supernatant (PgSup) into S. pneumoniae-infected mice. Mice were intratracheally injected with S. pneumoniae and PgSup to induce pneumonia, and lung histopathological sections and the absolute number and frequency of neutrophils and macrophages in the lung were analyzed. Proinflammatory cytokine/chemokine expression was examined by qPCR and ELISA. Inflammatory cell infiltration was observed in S. pneumoniae-infected mice and S. pnemoniae and PgSup mixed-infected mice, and mixed-infected mice showed more pronounced inflammation in lung. The ratios of monocytes/macrophages and neutrophils were not significantly different between the lungs of S. pneumoniae-infected mice and those of mixed-infected mice. PgSup synergistically increased TNF-α expression/production and IL-17 production compared with S. pneumoniae infection alone. We demonstrated that PgSup enhanced inflammation in pneumonia caused by S. pneumoniae, suggesting that virulence factors produced by P. gingivalis are involved in the exacerbation of respiratory tract infections such as aspiration pneumonia.
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DOI：
10.3390/jof3040052
发表时间：
2017-09-27
期刊：
Journal of fungi (Basel, Switzerland)
影响因子：
--
作者：
Mengesha BG;Conti HR
通讯作者：
Conti HR
DOI：
10.1165/rcmb.2011-0011rt
发表时间：
2011-08-01
影响因子：
6.4
作者：
Parker, Dane;Prince, Alice
通讯作者：
Prince, Alice
DOI：
10.4049/jimmunol.1600138
发表时间：
2016-09-15
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Liu R;Lauridsen HM;Amezquita RA;Pierce RW;Jane-Wit D;Fang C;Pellowe AS;Kirkiles-Smith NC;Gonzalez AL;Pober JS
通讯作者：
Pober JS
影响因子：
4.2
作者：
Johnson, JL;Hirsch, CS
通讯作者：
Hirsch, CS
影响因子：
2.6
作者：
Hasegawa, Y;Nishiyama, S;Yoshimura, F
通讯作者：
Yoshimura, F