Molecular characterization of airborne fungi in caves of the Mogao Grottoes, Dunhuang, China

Molecular characterization of airborne fungi in caves of the Mogao Grottoes, Dunhuang, China
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中国敦煌莫高窟洞穴中空气真菌的分子特征

DOI:
10.1016/j.ibiod.2011.04.006
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发表时间:
2011-08
影响因子:
4.8
通讯作者:
Ma, Yantian
Ma, Yantian
中科院分区:
环境科学与生态学2区
文献类型:
--
作者:
Feng, Huyuan;Ma, Xiaojun;Wu, Fasi;Ma, Xu;Wang, Wanfu;An, Lizhe;Mao, Lin;Ma, Yantian

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在这项研究中,我们分析了空气样品收集的几个地点在莫高窟,敦煌,中国。从2008年9月至2009年8月,每个月从莫高窟的一个开放洞(OC)、一个半开放洞(SC)、一个封闭洞(CC)和一个入口(EN)采集样品。使用六阶段Andersen FA-I采样器进行采样;然后培养样品,并通过其内部转录间隔区(ITS)区域的部分测序鉴定真菌分离株。共发现11个真菌属,其中枝孢属最多,其次为镰刀菌属、青霉属、交链孢属和曲霉属。真菌群落组成在四个站点之间存在差异。真菌群落结构与地点(r=-0.293,p = 0.039)和季节(r=-0.523,p = 0.000)显著相关。空气中真菌的浓度和丰度在四个采样点全年变化很大。气象参数(例如,温度、相对湿度)和游客数量也影响了莫高窟气传真菌的丰度和群落结构。
In this study, we analyzed air samples collected from several sites within the Mogao Grottoes, Dunhuang, China. The samples were collected each month from September 2008 to August 2009 from an open cave (OC), a semi-open cave (SC), a closed cave (CC), and the entrance (EN) of the Mogao Grottoes. Sampling was carried out using a six-stage Andersen FA-I sampler; then samples were cultured and fungal isolates were identified by partial sequencing of their internal transcribed spacer (ITS) region. Eleven different fungal genera were found, and the most prevalent wasCladosporium, followed byFusarium,Penicillium,Alternaria, andAspergillus. The fungal community composition varied among the four sites. Fungal community structure was significantly related to site (r= −0.293,p= 0.039) and to time of year (r= −0.523,p= 0.000). The concentrations and abundance of airborne fungi varied greatly throughout the year at the four sampling sites. Meteorological parameters (e.g., temperature, relative humidity) and the number of visitors also influenced both abundance and community structure of airborne fungi in the Mogao Grottoes.
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