Systematic identification of fragile sites via genome-wide location analysis of gamma-H2AX.
Systematic identification of fragile sites via genome-wide location analysis of gamma-H2AX.
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DOI：
10.1038/nsmb.1754
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发表时间：
2010-03
影响因子：
16.8
通讯作者：
中科院分区：
文献类型：
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Phosphorylation of histone H2AX is an early response to DNA damage in eukaryotes. In Saccharomyces cerevisiae, DNA damage or replication fork stalling results in histone H2A phosphorylation to yield γ-H2A (yeast γ-H2AX) in a Mec1 (ATR)- and Tel1 (ATM)- dependent manner. Here, we describe the genome-wide location analysis of γ-H2A as a strategy to identify loci prone to engage the Mec1 and Tel1 pathways. Remarkably, γ-H2A enrichment overlaps with loci prone to replication fork stalling and is caused by the action of Mec1 and Tel1, indicating that these loci are prone to breakage. Moreover, about half the sites enriched for γ-H2A map to repressed protein-coding genes, and histone deacetylases are necessary for formation of γ-H2A at these loci. Finally, our work indicates that high resolution mapping of γ-H2AX is a fruitful route to map fragile sites in eukaryotic genomes.
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影响因子：
10.5
作者：
Admire, A;Shanks, L;Weinert, T
通讯作者：
Weinert, T
影响因子：
16
作者：
Ivessa, AS;Lenzmeier, BA;Zakian, VA
通讯作者：
Zakian, VA
在酵母和哺乳动物中，异染色质对 γ-H2AX 修饰具有抵抗力。
DOI：
10.1083/jcb.200612031
发表时间：
2007-07-16
期刊：
The Journal of cell biology
影响因子：
--
作者：
Kim JA;Kruhlak M;Dotiwala F;Nussenzweig A;Haber JE
通讯作者：
Haber JE
影响因子：
10.5
作者：
Azvolinsky, Anna;Dunaway, Stephen;Zakian, Virginia A.
通讯作者：
Zakian, Virginia A.
影响因子：
64.8
作者：
Downs, JA;Lowndes, NF;Jackson, SP
通讯作者：
Jackson, SP