Composition and effect of anti-hypoxia of Chinese medicinal plant mixture

Composition and effect of anti-hypoxia of Chinese medicinal plant mixture
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中药植物合剂的组成及抗缺氧作用

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2011
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研究了参降所养益气药(SJSY,中草药合剂)的体内外抗缺氧活性。HPLC-DAD法测定了人参皂苷rg1、淫羊藿苷和芍药苷的含量。采用MTT法对两种细胞系(Vero和BHK-21)进行体外抗缺氧活性评价。与对照组(Vero: 48.72%, BHK-21: 35.41%)相比,高剂量组细胞存活率分别为87.33% (Vero)和83.03% (BHK-21)。流式细胞仪检测混合物对缺氧条件下细胞周期G1期阻滞的抑制作用。采用小鼠模型研究了体内抗缺氧活性。
The in vitro and in vivo anti-hypoxia activity of shenjiangsuoyangyiqi drug (SJSY, Chinese medicinal plant mixture) was investigated. The compounds ginsenoside rg1, icariin and peoniflorin were detected in mixture by HPLC-DAD. In vitro anti-hypoxia activity of the mixture was evaluated for MTT assay against two cell lines (Vero and BHK-21). Compared to the control group (Vero: 48.72% and BHK-21: 35.41%), the survival rates of both cells were increased to 87.33% (Vero) and 83.03% (BHK-21) in highdoes group. Arresting of the G1 phase of the cell-cycle under hypoxic condition was inhibited by mixture which was analyzed by FCM. In vivo anti-hypoxia activity was studied in mice model.
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