Extended expression of B-class MADS-box genes in the paleoherb Asarum caudigerum.
Extended expression of B-class MADS-box genes in the paleoherb Asarum caudigerum.
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B 类 MADS-box 基因在古草本细辛中的扩展表达
DOI：
10.1007/s00425-009-1048-6
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发表时间：
2010-01
期刊：
影响因子：
4.3
通讯作者：
Li DZ
中科院分区：
文献类型：
--
作者：
Zhao YH;Möller M;Yang JB;Liu TS;Zhao JF;Dong LN;Zhang JP;Li CY;Wang GY;Li DZ
细辛属(Aristolochiaceae)细辛是一种古草本植物，在被子植物系统发育中处于基础地位，对被子植物花的起源和进化研究具有重要意义。本研究利用抑制性消减杂交技术，构建了紫云英花蕾消减文库，构建了消减文库，构建了消减文库。利用了开花前不同时期的花蕾和苗期叶片的cDNA。对分离的花基因表达谱的大阵列分析表明，在所测试的612个唯一EST中，有157个基因在花芽和上部叶片中表现出较高的转录丰度。其中78个基因在花被中差异表达，62个基因在雄蕊中差异表达，100个基因在心皮中差异表达。所选基因的实时定量聚合酶链式反应验证了大芯片的结果。在花被、雄蕊和心皮中，分离到的APETALA3(AP3)B类基因在花被、雄蕊和心皮中表达上调，这意味着B类基因在这种基本被子植物中的表达范围比它们在优生植物中的表达区域更广。
Asarum caudigerum (Aristolochiaceae) is a paleoherb species that is important for research in origin and evolution of angiosperm flowers due to its basal position in the angiosperm phylogeny. In this study, a subtracted floral cDNA library from floral buds of A. caudigerum was constructed and cDNA arrays by suppression subtractive hybridization were generated. cDNAs of floral buds at different stages before flower opening and of leaves at the seedling stage were used. The macroarray analyses of expression profiles of isolated floral genes showed that 157 genes out of the 612 unique ESTs tested revealed higher transcript abundance in the floral buds and uppermost leaves. Among them, 78 genes were determined to be differentially expressed in the perianth, 62 in the stamens, and 100 genes in the carpels. Quantitative real-time PCR of selected genes validated the macroarray results. Remarkably, APETALA3 (AP3) B-class genes isolated from A. caudigerum were upregulated in the perianth, stamens and carpels, implying that the expression domain of B-class genes in this basal angiosperm was broader than those in their eudicot counterparts.
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影响因子：
2.3
作者：
Kramer, EM;Di Stilio, VS;Schl端ter, PM
通讯作者：
Schl端ter, PM
影响因子：
64.5
作者：
JACK, T;BROCKMAN, LL;MEYEROWITZ, EM
通讯作者：
MEYEROWITZ, EM
影响因子：
64.5
作者：
DREWS, GN;BOWMAN, JL;MEYEROWITZ, EM
通讯作者：
MEYEROWITZ, EM
影响因子：
11.4
作者：
Egea-Cortines, M;Saedler, H;Sommer, H
通讯作者：
Sommer, H
影响因子：
2.9
作者：
Jaramillo, MA;Kramer, EM
通讯作者：
Kramer, EM