Phospholipid bilayer affinities and solvation characteristics by electrokinetic chromatography with a nanodisc pseudostationary phase.
Phospholipid bilayer affinities and solvation characteristics by electrokinetic chromatography with a nanodisc pseudostationary phase.
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DOI：
10.1002/elps.201600381
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发表时间：
2017-03
期刊：
影响因子：
2.9
通讯作者：
Palmer CP
中科院分区：
文献类型：
--
作者：
Penny WM;Steele HB;Ross JB;Palmer CP
由1，2-二肉豆蔻基-3-甘油-3-磷胆碱和人工合成的马来酸-苯乙烯共聚物带组成的磷脂双层纳米盘在电动色谱中被引入作为拟固定相(PSP)，表现出良好的性能。纳米盘提供了合适的迁移范围和较高的理论板数。利用该纳米盘PSP，测定和表征了双层结构对探针溶质的亲和力。对于特定类别的溶质，保留因子和辛醇水分配系数之间有很好的相关性，但总体相关性较弱，主要是因为纳米盘对氢键给体的亲和力强于辛醇。这表明，这项技术的更合适的应用是直接测量和表征溶质和脂双层之间的相互作用。本研究中纳米盘-溶质相互作用的线性溶剂化能关系分析表明，纳米盘提供了一个低内聚性和弱氢键给予能力的溶剂化环境，并提供了相对较强的氢键受体强度。
Phospholipid bilayer nanodiscs composed of 1,2-dimyristoyl-sn-glycero-3-phosphocholine and synthetic maleic acid-styrene copolymer belts have been introduced as a pseudostationary phase (PSP) in electrokinetic chromatography and demonstrated good performance. The nanodiscs provide a suitable migration range and high theoretical plate counts. Using this nanodisc PSP, the affinity of the bilayer structure for probe solutes was determined and characterized. Good correlation is observed between retention factors and octanol water partition coefficients for particular categories of solutes, but the general correlation is weak primarily because the nanodiscs show stronger affinity than octanol for hydrogen bond donors. This suggests that a more appropriate application of this technology is to measure and characterize interactions between solutes and lipid bilayers directly. Linear solvation energy relationship analysis of the nanodisc-solute interactions in this study demonstrates that the nanodiscs provide a solvation environment with low cohesivity and weak hydrogen bond donating ability, and provide relatively strong hydrogen bond acceptor strength.
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