Three-dimensional structure of T3 connector purified from overexpressing bacteria.
Three-dimensional structure of T3 connector purified from overexpressing bacteria.
复制标题
从过度表达的细菌中纯化的 T3 连接器的三维结构。
DOI：
10.1016/0022-2836(92)90579-9
复制
发表时间：
1992
影响因子：
5.6
通讯作者：
JoséL. Carrascosa
中科院分区：
文献类型：
--
作者：
JoséMaría Valpuesta;Hisao Fujisawa;Sergio Marco;JoséM. Carazo;JoséL. Carrascosa
噬菌体T3连接子已从大肠杆菌中过表达的蛋白质中纯化，其携带含有编码p8蛋白的基因的质粒。连接器，这是由12个拷贝的p8，已结晶在二维片和负染色标本的电子显微镜研究。一个二维傅立叶滤波和平均程序进行与晶体标本。此外，单颗粒平均技术与其他制剂一起使用。从这两种方法获得的平均图像给出了类似的结果。通过收集几组倾斜视图并使用标准傅立叶程序，从T3连接器的二维晶体获得三维重建。三维图谱的分辨率为1.65 nm。重建的连接器显示了两个主要领域：一个更宽的12个小单元的外围和14.9 nm的外径。和直径为8.5nm的较小的一个。重建的连接器的高度已被确定为约8.5 nm。重建清楚地显示了内部开放通道，其沿着颗粒的纵轴延伸并且具有3.7 nm的平均直径。
The bacteriophage T3 connector has been purified from overexpressed protein inEscherichia coli, harboring a plasmid containing the gene encoding p8 protein. The connector, which is composed of 12 copies of p8, has been crystallized in two-dimensional sheets and studied by electron microscopy from negatively stained specimens. A two-dimensional Fourier filtering and averaging procedure was performed with crystalline specimens. In addition, single particle averaging techniques were used with other preparations. The average images obtained from these two approaches gave similar results. A three-dimensional reconstruction from two-dimensional crystals of T3 connectors was obtained by collecting several sets of tilted views and using standard Fourier procedures. The resolution of the three-dimensional map was 1.65 nm. The reconstructed connector shows two main domains: a wider one with 12 small units in the periphery and with an external diameter of 14.9 nm. and a smaller one with 8.5 nm diameter. The height of the reconstructed connector has been determined to be around 8.5 nm. The reconstruction clearly shows an internal open channel running along the longitudinal axis of the particle and having an average diameter of 3.7 nm.
从基因 2.5 到基因 9 的噬菌体 T3 DNA 序列。
影响因子：
5.6
作者：
Beck,PJ;Gonzalez,S;Ward,CL;Molineux,IJ
通讯作者：
Molineux,IJ
通过电子显微镜和计算机图像处理研究核糖体结构。
影响因子：
--
作者：
Frank,J;Radermacher,M;Wagenknecht,T;Verschoor,A
通讯作者：
Verschoor,A