Excessive intrauterine fluid cause aberrant implantation and pregnancy outcome in mice.
Excessive intrauterine fluid cause aberrant implantation and pregnancy outcome in mice.
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子宫内液体过多导致小鼠异常着床和妊娠结果
DOI：
10.1371/journal.pone.0078446
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Qiao J
中科院分区：
文献类型：
--
作者：
Lu S;Peng H;Zhang H;Zhang L;Cao Q;Li R;Zhang Y;Yan L;Duan E;Qiao J
正常的子宫内液体环境对胚胎着床至关重要。输卵管积水患者行IVF-胚胎移植后，种植率和妊娠率明显下降，而输卵管切除术可明显提高妊娠率。输卵管积水渗漏到子宫内膜腔内是影响生育力的主要原因。然而，输卵管积水对着床和持续妊娠的潜在机制尚未完全了解，关于其毒性仍存在争议。在本研究中，通过在小鼠胚胎植入前（第4天08：30）将不同体积的无毒液体（0.9%生理盐水）注入子宫腔，我们发现，虽然胚胎没有从子宫中“冲出”，但植入时间推迟，正常的宫内分布（胚胎间距）被破坏。妊娠早期的异常着床进一步导致胚胎生长迟缓、流产和妊娠丢失增加，这与在接受IVF-ET的输卵管积水患者中观察到的不良反应相似。我们进一步检测了子宫容受性相关基因表达，这些基因与人类输卵管积水有关（Lif、Hoxa 10、整合素α（v）和β（3））。结果表明，子宫液灌注后整合素α（v）和β（3）表达增加，提示子宫液灌注后对过度体液环境的代偿作用。我们的数据表明，过量的无毒腔液对妊娠的不良影响主要是由于机械干扰了正常的胚胎贴壁时间和位置，这可能是IVF-ET合并输卵管积水患者种植率下降的主要原因。
The normal intrauterine fluid environment is essential for embryo implantation. In hydrosalpinx patients, the implantation and pregnancy rates are markedly decreased after IVF–embryo transfer, while salpingectomy could significantly improve the pregnancy rates. The leakage of hydrosalpinx fluid into the endometrial cavity was supposed to be the major cause for impaired fertility. However, the underlying mechanisms of hydrosalpinx fluids on implantation and ongoing pregnancy were not fully understood and remain controversial regarding its toxicity. In present study, by infusing different volume of non-toxic fluid (0.9% saline) into uterine lumen before embryo implantation in mice (Day4 08:30), we found that while the embryos were not “flushed out” from the uteri, the timing of implantation was deferred and normal intrauterine distribution (embryo spacing) was disrupted. The abnormal implantation at early pregnancy further lead to embryo growth retardation, miscarriage and increased pregnancy loss, which is similar to the adverse effects observed in hydrosalpinx patients undergoing IVF-ET. We further examined uterine receptivity related gene expression reported to be involved in human hydrosalpinx (Lif, Hoxa10, Integrin α(v) and β(3)). The results showed that expression of integrin α(v) and β(3) were increased in the fluid infused mouse uteri, implicating a compensatory effect to cope with the excessive fluid environment. Our data suggested that the adverse effects of excessive non-toxic luminal fluid on pregnancy are primarily due to the mechanical interference for normal timing and location of embryo apposition, which might be the major cause of decreased implantation rate in IVF-ET patients with hydrosalpinx.
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DOI：
10.1074/jbc.m110.197202
发表时间：
2011-02-11
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Chen Q;Zhang Y;Peng H;Lei L;Kuang H;Zhang L;Ning L;Cao Y;Duan E
通讯作者：
Duan E
影响因子：
6.1
作者：
Eytan, O;Azem, F;Jaffa, AJ
通讯作者：
Jaffa, AJ
影响因子：
4
作者：
CLEMETSO.CA;MALLIKAR.VR;WILDS, JH
通讯作者：
WILDS, JH
影响因子：
3.8
作者：
Naftalin, RJ;Thiagarajah, JR;Milligan, SR
通讯作者：
Milligan, SR
影响因子：
6.1
作者：
Camus, E;Poncelet, C;Philippe, HJ
通讯作者：
Philippe, HJ