Engaging Cold to Upregulate Cell Proliferation in Alginate-Encapsulated Liver Spheroids.
Engaging Cold to Upregulate Cell Proliferation in Alginate-Encapsulated Liver Spheroids.
复制标题
利用冷来上调藻酸盐包裹的肝球体中的细胞增殖。
DOI：
10.1089/ten.tec.2017.0131
复制
发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Kilbride P
中科院分区：
文献类型：
--
作者：
Kilbride P
多年来，研究人员一直在研究高温和低温对哺乳动物细胞的影响。除了在移植或再生医学保存等领域中使用的短期低温储存外，先前尚未报道在肝细胞中使用低温处理的优点。我们已经观察到，与未处理的样品相比，冷冻保存或经历温度冷降低（≤10 ℃）1至90分钟的藻酸盐包封的HepG 2肝球状体在处理后7-16天的培养期间显示出增强的细胞增殖。在处理后8-12天后，藻酸盐包封的肝球状体经历了对照样品的1.71 ± 0.35倍的细胞密度（p< 0.001）。这种效应发生在各种冷处理的样品中。这种低温处理提供了一种简单的方法来快速提高细胞增殖率的扩展培养系统，如生物人工肝装置。这将允许更快速地制造所需的生物质，并达到更高的细胞密度，从而减少最终所需的生物质体积。这可以使生物人工肝设备更便宜地制备，使其成为更具成本效益的治疗方法。
For many years, the impact of hyper- and hypothermia on mammalian cells has been examined. With the exception of short, low temperature storage, which has uses in areas such as preservation for transplantation or regenerative medicine, advantages for the use of low temperature treatment in hepatocytes have not been previously reported. We have observed that alginate-encapsulated HepG2 liver spheroids that are cryopreserved or experience a cold reduction in temperature (≤10°C) for periods between 1 and 90 min display an enhanced cell proliferation during culture 7–16 days post-treatment compared with untreated samples. Following 8–12 days post-treatment, alginate-encapsulated liver spheroids experienced a cell density of 1.71 ± 0.35 times that of control samples (p< 0.001). This effect occurred in samples with a variety of cold treatments. This low temperature treatment offers a simple method to rapidly increase cell proliferation rates for extended culture systems, such as bioartificial liver devices. This would allow the manufacture of required biomass more rapidly, and to a higher cell density, reducing final required biomass volume. This could enable bioartificial liver devices to be prepared more cheaply, making them a more cost effective treatment.
登录
查看更多内容
影响因子：
3
作者：
Y. Bachiri;C. Gazeau;J. Hansz;C. Morisset;J. Dereuddre
通讯作者：
J. Dereuddre
影响因子：
3
作者：
Massie, Isobel;Selden, Clare;Fuller, Barry
通讯作者：
Fuller, Barry
冷休克下人类滑膜细胞中钙依赖性 DNA 断裂
影响因子：
3.5
作者：
M. Perotti;Filippo Toddei;F. Mirabelli;M. Vairetti;Giorgo Bellomo;D. McConkey;S. Orrenius
通讯作者：
S. Orrenius
影响因子：
3
作者：
T. West;M. Ravindra;J. Preece
通讯作者：
J. Preece
DOI：
--
发表时间：
1987
期刊：
影响因子：
--
作者：
B. Grout;G. J. Morris
通讯作者：
G. J. Morris