Monoclonal antibodies to cytomegalovirus: rapid identification of clinical isolates and preliminary use in diagnosis of cytomegalovirus pneumonia
Monoclonal antibodies to cytomegalovirus: rapid identification of clinical isolates and preliminary use in diagnosis of cytomegalovirus pneumonia
复制标题
巨细胞病毒单克隆抗体：临床分离株的快速鉴定及其在巨细胞病毒肺炎诊断中的初步应用
作者：
L. Goldstein;'. J. McDOUGALL;R. Hackman;D. Joel;Meyers;E.;D. Thomas;R. Nowinski
描述了两种与巨细胞病毒（CMV）感染的细胞特异性反应的单克隆抗体。一种抗体6-E3与一种72，000-道尔顿的蛋白质反应，这种蛋白质在感染早期出现，并保持在细胞核中。另一种抗体6-C5与80，000-道尔顿的蛋白质反应，该蛋白质在感染后期出现并保持定位于细胞质包涵体中。这两种单克隆抗体均与常规实验室CMV菌株反应，并可用于免疫荧光测定，以鉴定培养物中的CMV临床分离株。对CMV肺炎患者肺组织的初步检测显示，仅抗体6-C5可检测到初级临床标本中的CMV感染。培养，组织学和免疫学方法的比较表明，单克隆抗体具有足够的特异性和灵敏度，以保证其作为免疫诊断工具，用于检测CMV感染的组织培养和直接从患者获得的组织中的持续发展。
Two monoclonal antibodies which react specifically with cells infected by cytomegalovirus (CMV) are described. One antibody, 6-E3, reacts with a 72,000-dalton protein that appears early in infection and remains localized in the cell nucleus. The other antibody, 6-C5, reacts with an 80,000-dalton protein that appears late in infection and remains localized in cytoplasmic inclusion bodies. Both monoclonal antibodies react with conventional laboratory strains of CMV and can be used in immunofluorescence assays to identify clinical isolates of CMV in culture. Preliminary tests on lung tissues from patients with CMV pneumonia show that only antibody 6-C5 detects CMV infection in primary clinical specimens. A comparison of culture, histological, and immunological methods demonstrates that the monoclonal antibodies possess sufficient specificity and sensitivity to warrant their continued development as immunodiagnostic tools for the detection of CMV infection in both tissue culture and tissues obtained directly from patients.
通过开肺活检的接触印迹快速细胞学诊断巨细胞病毒间质性肺炎。
影响因子：
3.5
作者：
Shulman,HM;Hackman,RC;Sale,GE;Meyers,JD
通讯作者：
Meyers,JD