Yeast phosphatidylinositol 4-kinase, Pik1, has essential roles at the Golgi and in the nucleus.
Yeast phosphatidylinositol 4-kinase, Pik1, has essential roles at the Golgi and in the nucleus.
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酵母磷脂酰肌醇4-激酶PIK1在高尔基体和细胞核中具有重要作用。
DOI：
10.1083/jcb.200504104
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发表时间：
2005-12-19
影响因子：
7.8
通讯作者：
Thorner, J
中科院分区：
文献类型：
--
作者：
Strahl, T;Hama, H;DeWald, DB;Thorner, J
磷脂酰肌醇4-激酶，Pik 1，是生存所必需的。GFP-Pik 1定位于细胞质点和细胞核。斑点与反式高尔基体池的标记物Sec 7-DsRed共定位。Kap 95（importin-β）是核入境所必需的，但Kap 60（importin-α）不是，而输出Msn 5是核出境所必需的。Frq 1（frequenin直系同源物）也是生存力所必需的，并在Pik 1的NH 2末端附近结合。Frq 1-GFP定位于高尔基体点，缺乏其Frq 1结合位点的Pik 1（或在frq 1 Δ细胞中过表达的Pik 1）不装饰高尔基体，但核定位未受干扰。缺乏核输出序列的Pik 1（Δ10-192）显示出显著的核积聚，并且不能挽救pik 1 Δ细胞的失活。Pik 1-CCAAX嵌合体被从细胞核中排除，也没有挽救pik 1 Δ细胞的失活。然而，Pik 1（Δ10-192）和Pik 1-CCAAX在pik 1 Δ细胞中的共表达恢复了活力。这些隔室限制的Pik 1结构的无催化活性的衍生物表明，PtdIns 4P必须在细胞核和高尔基体中产生正常的细胞功能。
Phosphatidylinositol 4-kinase, Pik1, is essential for viability. GFP-Pik1 localized to cytoplasmic puncta and the nucleus. The puncta colocalized with Sec7-DsRed, a marker of trans-Golgi cisternae. Kap95 (importin-β) was necessary for nuclear entry, but not Kap60 (importin-α), and exportin Msn5 was required for nuclear exit. Frq1 (frequenin orthologue) also is essential for viability and binds near the NH2 terminus of Pik1. Frq1-GFP localized to Golgi puncta, and Pik1 lacking its Frq1-binding site (or Pik1 overexpressed in frq1Δ cells) did not decorate the Golgi, but nuclear localization was unperturbed. Pik1(Δ10-192), which lacks its nuclear export sequence, displayed prominent nuclear accumulation and did not rescue inviability of pik1Δ cells. A Pik1-CCAAX chimera was excluded from the nucleus and also did not rescue inviability of pik1Δ cells. However, coexpression of Pik1(Δ10-192) and Pik1-CCAAX in pik1Δ cells restored viability. Catalytically inactive derivatives of these compartment-restricted Pik1 constructs indicated that PtdIns4P must be generated both in the nucleus and at the Golgi for normal cell function.
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影响因子：
7.8
作者：
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通讯作者：
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