Spatial and temporal characteristics of normal and perturbed vesicle transport.
Spatial and temporal characteristics of normal and perturbed vesicle transport.
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DOI：
10.1371/journal.pone.0097237
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Gunawardena S
中科院分区：
文献类型：
--
作者：
Iacobucci GJ;Rahman NA;Valtueña AA;Nayak TK;Gunawardena S
有效的细胞内转运对于健康的细胞功能和结构完整性是必不可少的，而这一途径中的问题可能会导致神经细胞死亡和疾病。为了在空间和时间上评估运输缺陷是如何启动的，我们采用了来自果蝇幼虫大脑的原代神经元培养系统来可视化几个货物/细胞器随着时间的推移沿着90微米轴突的运动动力学。所有6个囊泡/细胞器在第1天和第2天都显示出强大的双向运动。运动蛋白的减少减少了囊泡/细胞器的运动，并增加了神经突起块的数量。神经元的生长也因运动蛋白的减少而受到干扰。引人注目的是，我们发现并不是所有的阻塞都像之前认为的那样是固定的、永久性的阻塞，阻碍了囊泡的运输，而是一些阻塞是动态的囊泡簇，随着时间的推移而消失。综上所述，我们的发现表明，非解决性障碍可能会启动导致死亡和退化的有害途径，而解决性障碍可能是良性的。因此，评估囊泡运输的时空特征对于我们理解运输缺陷如何影响其他途径来启动死亡和退化具有重要的意义。
Efficient intracellular transport is essential for healthy cellular function and structural integrity, and problems in this pathway can lead to neuronal cell death and disease. To spatially and temporally evaluate how transport defects are initiated, we adapted a primary neuronal culture system from Drosophila larval brains to visualize the movement dynamics of several cargos/organelles along a 90 micron axonal neurite over time. All six vesicles/organelles imaged showed robust bi-directional motility at both day 1 and day 2. Reduction of motor proteins decreased the movement of vesicles/organelles with increased numbers of neurite blocks. Neuronal growth was also perturbed with reduction of motor proteins. Strikingly, we found that all blockages were not fixed, permanent blocks that impeded transport of vesicles as previously thought, but that some blocks were dynamic clusters of vesicles that resolved over time. Taken together, our findings suggest that non-resolving blocks may likely initiate deleterious pathways leading to death and degeneration, while resolving blocks may be benign. Therefore evaluating the spatial and temporal characteristics of vesicle transport has important implications for our understanding of how transport defects can affect other pathways to initiate death and degeneration.
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DOI：
10.1002/neu.20230
发表时间：
2006-03-01
期刊：
JOURNAL OF NEUROBIOLOGY
影响因子：
--
作者：
Baqri, R;Charan, R;Ray, K
通讯作者：
Ray, K
影响因子：
3.3
作者：
Barkus, Rosemarie V.;Klyachko, Olga;Saxton, William M.
通讯作者：
Saxton, William M.
影响因子：
16.2
作者：
BROADIE, K;PROKOP, A;SWEENEY, ST
通讯作者：
SWEENEY, ST
影响因子：
3.3
作者：
Brendza, RP;Sheehan, KB;Saxton, WM
通讯作者：
Saxton, WM
DOI：
10.1083/jcb.141.2.443
发表时间：
1998-04-20
期刊：
The Journal of cell biology
影响因子：
--
作者：
Gindhart JG Jr;Desai CJ;Beushausen S;Zinn K;Goldstein LS
通讯作者：
Goldstein LS