Identification of maturation and protein synthesis related proteins from porcine oocytes during in vitro maturation.
Identification of maturation and protein synthesis related proteins from porcine oocytes during in vitro maturation.
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DOI：
10.1186/1477-5956-9-28
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发表时间：
2011-06-08
期刊：
影响因子：
2
通讯作者：
Chae JI
中科院分区：
文献类型：
--
作者：
Kim J;Kim JS;Jeon YJ;Kim DW;Yang TH;Soh Y;Lee HK;Choi NJ;Park SB;Seo KS;Chung HM;Lee DS;Chae JI
哺乳动物卵母细胞的体外成熟（IVM）分为GV（萌发囊泡期）、MI（中期I期）和MII（中期II期）三个阶段，只有完全成熟的卵母细胞才具有受精和启动合子发育的能力。这些观察主要是基于形态学的评价，但这些过程的分子事件并没有完全理解。本研究的目的是更好地了解参与IVM的分子调控的过程中使用2-DE分析，然后通过质谱法，以确定在卵母细胞IVM差异表达的蛋白质。共鉴定了16个上调和12个下调的蛋白质。为了研究IVM过程，我们特别关注与GV期相比在MII期上调的蛋白质，包括PRDX 2、GST、SPSY、myomegalin、PED 4D、PRKAB 1和DTNA。这些上调的蛋白质在功能上参与氧化还原调节和cAMP依赖性途径，这对于参与卵母细胞成熟的细胞内信号传导是必需的。有趣的是，PDE 4D和它的伴侣，肌巨蛋白，在MII阶段，一致证实上调蛋白质印迹分析。这些结果可用于更好地了解猪卵母细胞成熟的分子机制的某些方面。本研究确定了一些调节蛋白，可能有重要的作用，在猪卵母细胞成熟的分子事件，特别是在卵母细胞减数分裂恢复，MII停滞和卵母细胞激活的调节。此外，这项研究可能有有益的应用，不仅在基础科学方面的卵母细胞培养条件的改善，但也哺乳动物生殖生物技术的潜在影响。
In vitro maturation (IVM) of mammalian oocytes is divided into the GV (germinal vesicle stage), MI (metaphase I stage) and MII (metaphase II stage) stages, and only fully mature oocytes have acquired the ability to be fertilized and initiate zygotic development. These observations have been mostly based on morphological evaluations, but the molecular events governing these processes are not fully understood. The aim of the present study was to better understand the processes involved in the molecular regulation of IVM using 2-DE analysis followed by mass spectrometry to identify proteins that are differentially expressed during oocyte IVM. A total of 16 up-regulated and 12 down-regulated proteins were identified. To investigate the IVM process, we specifically focused on the proteins that were up-regulated during the MII stage when compared with the GV stage, which included PRDX 2, GST, SPSY, myomegalin, PED4D, PRKAB 1, and DTNA. These up-regulated proteins were functionally involved in redox regulation and the cAMP-dependent pathway, which are essential for the intracellular signaling involved in oocyte maturation. Interestingly, the PDE4D and its partner, myomegalin, during the MII stage was consistently confirmed up-regulation by western blot analyses. These results could be used to better understand some aspects of the molecular mechanisms underlying porcine oocyte maturation. This study identified some regulatory proteins that may have important roles in the molecular events involved in porcine oocyte maturation, particularly with respect to the regulation of oocyte meiotic resumption, MII arrest and oocyte activation. In addition, this study may have beneficial applications not only to basic science with respect to the improvement of oocyte culture conditions but also to mammalian reproductive biotechnology with potential implications.
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影响因子：
2.5
作者：
Leyens, G;Knoops, B;Donnay, I
通讯作者：
Donnay, I
影响因子：
3.4
作者：
Berendt, Frank J.;Froehlich, Thomas;Arnold, Georg J.
通讯作者：
Arnold, Georg J.
影响因子：
2.8
作者：
Kim, J. S.;Cho, Y. S.;Koo, D. B.
通讯作者：
Koo, D. B.
DOI：
10.1530/jrf.0.1000403
发表时间：
1994-03-01
期刊：
JOURNAL OF REPRODUCTION AND FERTILITY
影响因子：
--
作者：
MATTIOLI, M;GALEATI, G;SEREN, E
通讯作者：
SEREN, E
影响因子：
4.8
作者：
Rabahi, F;Brûlé, S;Lussier, JG
通讯作者：
Lussier, JG