Au:CdHgTe quantum dots for in vivo tumor-targeted multispectral fluorescence imaging
Au:CdHgTe quantum dots for in vivo tumor-targeted multispectral fluorescence imaging
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用于体内肿瘤靶向多光谱荧光成像的 Au:CdHgTe 量子点
DOI：
10.1007/s00216-012-5921-y
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发表时间：
2012-03
影响因子：
4.3
通讯作者：
Jin, Wei1
中科院分区：
文献类型：
--
作者：
Han, Sihai1;Mu, Ying1;Zhu, Qiangyuan1;Gao, Yibo1;Li, Zuhong1;Jin, Qinhan1;Jin, Wei1
以L-谷胱甘肽和L-半胱氨酸为稳定剂，采用水溶液法制备了近红外金掺杂碲镉汞量子点（QDs），并对其光致发光性能和生物相容性进行了研究。制备的Au：CdHgTe量子点分别与精氨酸-甘氨酸-天冬氨酸（RGD）肽、抗表皮生长因子受体（EGFR）单克隆抗体（MAb）和抗癌胚抗原相关细胞粘附分子1（CEACAM 1）单克隆抗体（MAb）共价连接。三种Au：CdHgTe QD生物缀合物（QD 800-RGD、QD 820-anti-CEACAM 1和QD 840-anti-EGFR）成功地用作异种移植物的体内肿瘤靶向多光谱荧光成像的探针。将来自用于检测三种肿瘤标志物的QD生物缀合物的荧光信号光谱解混，并分析它们的共定位。结果表明，多光谱荧光成像可以同时检测多种肿瘤标志物在体内使用QD生物共轭物作为探针。这种方法具有良好的潜力，作为一种成像方法的非侵入性的探索和检测多种肿瘤标志物在体内，从而大大有助于癌症的诊断。
Near-infrared gold-doped CdHgTe quantum dots (QDs) with improved photoluminescence and biocompatibility were developed using an aqueous solution route with L-glutathione and L-cysteine as stabilizers. As-prepared Au:CdHgTe QDs were covalently linked to arginine-glycine-aspartic acid (RGD) peptide, anti-epidermal growth factor receptor (EGFR) monoclonal antibody (MAb), and anti- carcinoembryonic antigen-related cell adhesion molecule-1 (CEACAM1) MAb separately. Three Au:CdHgTe QD bioconjugates (QD800-RGD, QD820-anti-CEACAM1, and QD840-anti-EGFR) were successfully used as probes for in vivo tumor-targeted multispectral fluorescence imaging of xenografts. Fluorescence signals from the QD bioconjugates used to detect three tumor markers were spectrally unmixed, and their co-localization was analyzed. The results indicate that multiple tumor markers could be simultaneously detected by multispectral fluorescence imaging in vivo using QD bioconjugates as probes. This approach has excellent potential as an imaging method for the noninvasive exploration and detection of multiple tumor markers in vivo, thereby substantially aiding the diagnosis of cancer.
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影响因子：
18.3
作者：
Smith AM;Nie S
通讯作者：
Nie S
影响因子：
3.3
作者：
Qian, HF;Qiu, X;Ren, JC
通讯作者：
Ren, JC
影响因子：
--
作者：
Zhuang, JQ;Zhang, XD;Li, TJ
通讯作者：
Li, TJ
DOI：
10.1073/pnas.0610677104
发表时间：
2007-07-31
影响因子：
11.1
作者：
Vosch, Tom;Antoku, Yasuko;Dickson, Robert M.
通讯作者：
Dickson, Robert M.
影响因子：
2.8
作者：
J. Han;Lizhong Sun;X. Qu;Yuanping Chen;J. Zhong
通讯作者：
J. Han;Lizhong Sun;X. Qu;Yuanping Chen;J. Zhong