Loss of OLFM4 promotes tumor migration through inducing interleukin-8 expression and predicts lymph node metastasis in early gastric cancer.
Loss of OLFM4 promotes tumor migration through inducing interleukin-8 expression and predicts lymph node metastasis in early gastric cancer.
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OLFM4的缺失通过诱导白细胞介素8表达促进肿瘤迁移并预测早期胃癌的淋巴结转移
DOI：
10.1038/oncsis.2016.42
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发表时间：
2016-06-13
期刊：
影响因子：
6.2
通讯作者：
Qin X
中科院分区：
文献类型：
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作者：
Zhao J;Shu P;Duan F;Wang X;Min L;Shen Z;Ruan Y;Qin J;Sun Y;Qin X
内镜手术在全球范围内越来越多地用于早期胃癌（EGC）治疗，淋巴结转移仍然是EGC患者内镜手术的最重要风险因素。嗅觉调节蛋白4（OLFM 4）主要在消化系统中表达，并在几种类型的肿瘤中上调。然而，OLFM 4在EGC中的作用尚未被探索。我们通过免疫组织化学染色评估了105例接受胃切除术的EGC患者的OLFM 4表达。联合分析临床病理因素和OLFM 4表达对EGC淋巴结转移的预测作用。同时探讨OLFM 4在胃癌转移中的作用机制。我们发现OLFM 4在EGC肿瘤切片中上调，并且在淋巴结转移患者中观察到OLFM 4的相对低表达。OLFM 4表达、肿瘤大小和分化程度被确定为独立因素，可以将其联合分析以产生更好的预测EGC患者淋巴结转移的模型。体外研究表明，OLFM 4基因的敲除可通过激活NF-κB/IL-8轴促进胃癌细胞的迁移。在EGC肿瘤样品中也观察到OLFM 4和白细胞介素-8表达之间的负相关。我们的研究表明OLFM 4表达是EGC淋巴结转移的潜在预测因子，将OLFM 4与肿瘤大小和分化程度相结合可以更好地对具有不同淋巴结转移风险的EGC患者进行分层。
Endoscopic surgery is increasingly used for early gastric cancer (EGC) treatment worldwide, and lymph node metastasis remains the most important risk factor for endoscopic surgery in EGC patients. Olfactomedin 4 (OLFM4) is mainly expressed in the digestive system and upregulated in several types of tumors. However, the role of OLFM4 in EGC has not been explored. We evaluated OLFM4 expression by immunohistochemical staining in 105 patients with EGC who underwent gastrectomy. The clinicopathological factors and OLFM4 expression were co-analyzed to predict lymph node metastasis in EGC. The metastatic mechanism of OLFM4 in gastric cancer was also investigated. We found that OLFM4 was upregulated in EGC tumor sections, and relatively low expression of OLFM4 was observed in patients with lymph node metastasis. OLFM4 expression as well as tumor size and differentiation were identified as independent factors, which could be co-analyzed to generate a better model for predicting lymph node metastasis in EGC patients. In vitro studies revealed that knockdown of OLFM4 promoted the migration of gastric cancer cells through activating the NF-κB/interleukin-8 axis. Negative correlation between OLFM4 and interleukin-8 expression was also observed in EGC tumor samples. Our study implies that OLFM4 expression is a potential predictor of lymph node metastasis in EGC, and combing OLFM4 with tumor size and differentiation could better stratify EGC patients with different risks of lymph node metastasis.
影响因子：
--
作者：
Baik YH;An JY;Noh JH;Sohn TS;Kim S
通讯作者：
Kim S