ESKIMO1 disruption in Arabidopsis alters vascular tissue and impairs water transport.
ESKIMO1 disruption in Arabidopsis alters vascular tissue and impairs water transport.
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DOI：
10.1371/journal.pone.0016645
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发表时间：
2011-02-01
期刊：
影响因子：
3.7
通讯作者：
Durand-Tardif M
中科院分区：
文献类型：
--
作者：
Lefebvre V;Fortabat MN;Ducamp A;North HM;Maia-Grondard A;Trouverie J;Boursiac Y;Mouille G;Durand-Tardif M
农业实践中的水经济是一个正在通过旨在了解植物水利用效率（WUE）（即每消耗一次水产生的生物量）和对缺水的反应的研究来解决的问题。在模式物种拟南芥中，ESKIMO1（ESK1）基因被描述为参与抗冻性、耐寒性和耐盐性以及水分经济：ESK1突变体具有非常低的蒸散率和高的水分利用效率。为了建立ESK1功能，已经进行了ESK1突变体的详细表征。与野生型相比，esk 1中存在高水平的胁迫激素阿坝（脱落酸），然而，esk 1的弱水分损失不依赖于通过阿坝生物合成的气孔关闭，因为将该途径中的突变体与esk 1组合导致加性表型。根系导水率的测定表明esk 1营养器官由于水分运输的缺陷而遭受水分亏缺。在木质部和纤维中观察到ESK1启动子驱动的报告基因表达，木质部和纤维是负责通过根将水和矿物质营养从土壤运输到枝条的维管组织。此外，在下胚轴、根和茎的横切面上，esk 1木质部导管塌陷。最后，使用傅里叶变换红外（FTIR）光谱，严重的木质部细胞壁组成的化学修饰突出了esk1突变体。总之，我们的研究结果表明，ESK 1是必要的生产功能木质部导管，通过其含义在奠定了次生细胞壁组件。
Water economy in agricultural practices is an issue that is being addressed through studies aimed at understanding both plant water-use efficiency (WUE), i.e. biomass produced per water consumed, and responses to water shortage. In the model species Arabidopsis thaliana, the ESKIMO1 (ESK1) gene has been described as involved in freezing, cold and salt tolerance as well as in water economy: esk1 mutants have very low evapo-transpiration rates and high water-use efficiency. In order to establish ESK1 function, detailed characterization of esk1 mutants has been carried out. The stress hormone ABA (abscisic acid) was present at high levels in esk1 compared to wild type, nevertheless, the weak water loss of esk1 was independent of stomata closure through ABA biosynthesis, as combining mutant in this pathway with esk1 led to additive phenotypes. Measurement of root hydraulic conductivity suggests that the esk1 vegetative apparatus suffers water deficit due to a defect in water transport. ESK1 promoter-driven reporter gene expression was observed in xylem and fibers, the vascular tissue responsible for the transport of water and mineral nutrients from the soil to the shoots, via the roots. Moreover, in cross sections of hypocotyls, roots and stems, esk1 xylem vessels were collapsed. Finally, using Fourier-Transform Infrared (FTIR) spectroscopy, severe chemical modifications of xylem cell wall composition were highlighted in the esk1 mutants. Taken together our findings show that ESK1 is necessary for the production of functional xylem vessels, through its implication in the laying down of secondary cell wall components.
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影响因子：
5.3
作者：
Bouchabke-Coussa O;Quashie ML;Seoane-Redondo J;Fortabat MN;Gery C;Yu A;Linderme D;Trouverie J;Granier F;Téoulé E;Durand-Tardif M
通讯作者：
Durand-Tardif M
DOI：
10.1111/j.1755-0238.2002.tb00221.x
发表时间：
2002-01-01
影响因子：
2.8
作者：
Cholet, C;Mondolot, L;Andary, C
通讯作者：
Andary, C
影响因子：
7.4
作者：
Bischoff, Volker;Nita, Silvia;Scheible, Wolf-Ruediger
通讯作者：
Scheible, Wolf-Ruediger
影响因子：
7.2
作者：
Christmann, Alexander;Weiler, Elmar W.;Grill, Erwin
通讯作者：
Grill, Erwin
影响因子：
11.6
作者：
Javot, H;Lauvergeat, V;Maurel, C
通讯作者：
Maurel, C