ESKIMO1 is a key gene involved in water economy as well as cold acclimation and salt tolerance.
ESKIMO1 is a key gene involved in water economy as well as cold acclimation and salt tolerance.
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DOI：
10.1186/1471-2229-8-125
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发表时间：
2008-12-07
影响因子：
5.3
通讯作者：
Durand-Tardif M
中科院分区：
文献类型：
--
作者：
Bouchabke-Coussa O;Quashie ML;Seoane-Redondo J;Fortabat MN;Gery C;Yu A;Linderme D;Trouverie J;Granier F;Téoulé E;Durand-Tardif M
干旱是一个重大的社会和经济问题，导致农田产量大幅减少。今天的挑战是开发在波动的环境条件下具有降低的水需求和稳定的产量的植物。拟南芥是一个很好的模式，为确定潜在的目标植物育种。通过将该模式植物的基因转移到作物中，成功地赋予了作物田间耐旱性。虽然参与了植物基因组学计划，其目的是确定新的基因负责植物对非生物胁迫的反应，我们确定ESKIMO 1作为一个关键基因参与植物水分经济以及冷驯化和耐盐性。所有的esk1突变体更耐冷冻，驯化后，比野生型对应。esk 1突变体也表现出对轻度水分亏缺的所有测量性状的耐受性增加。突变体的耐受性提高，减少供水可能是由于其较低的蒸腾速率和更好的水分利用效率（WUE），这是由碳同位素歧视和气体交换测量评估。esk 1等位基因也显示出对盐胁迫更耐受。对一个突变系及其野生型背景进行了转录组学分析。在控制浇水条件下，突变体和野生型之间差异表达的基因的数量，而在轻度干旱胁迫下，这一名单的基因减少。在野生型和突变体之间差异表达的基因中，与对胁迫或生物和非生物刺激的响应相关的两个功能类别被过度代表。在盐胁迫条件下，所有的基因功能类别在突变体和野生型中表现相同。基于这种转录组分析，我们假设在对照条件下，esk1突变体的行为，如果它暴露于干旱胁迫。总体而言，我们的研究结果表明，ESKIMO1基因在植物响应缺水和整个植物水分经济中起着重要作用。正在进行进一步的实验来阐明ESKIMO1蛋白的功能及其调节植物水分吸收的方式。
Drought is a major social and economic problem resulting in huge yield reduction in the field. Today's challenge is to develop plants with reduced water requirements and stable yields in fluctuating environmental conditions. Arabidopsis thaliana is an excellent model for identifying potential targets for plant breeding. Drought tolerance in the field was successfully conferred to crops by transferring genes from this model species. While involved in a plant genomics programme, which aims to identify new genes responsible for plant response to abiotic stress, we identified ESKIMO1 as a key gene involved in plant water economy as well as cold acclimation and salt tolerance. All esk1 mutants were more tolerant to freezing, after acclimation, than their wild type counterpart. esk1 mutants also showed increased tolerance to mild water deficit for all traits measured. The mutant's improved tolerance to reduced water supply may be explained by its lower transpiration rate and better water use efficiency (WUE), which was assessed by carbon isotope discrimination and gas exchange measurements. esk1 alleles were also shown to be more tolerant to salt stress. Transcriptomic analysis of one mutant line and its wild-type background was carried out. Under control watering conditions a number of genes were differentially expressed between the mutant and the wild type whereas under mild drought stress this list of genes was reduced. Among the genes that were differentially expressed between the wild type and mutant, two functional categories related to the response to stress or biotic and abiotic stimulus were over-represented. Under salt stress conditions, all gene functional categories were represented equally in both the mutant and wild type. Based on this transcriptome analysis we hypothesise that in control conditions the esk1 mutant behaves as if it was exposed to drought stress. Overall our findings suggest that the ESKIMO1 gene plays a major role in plant response to water shortage and in whole plant water economy. Further experiments are being undertaken to elucidate the function of the ESKIMO1 protein and the way it modulates plant water uptake.
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影响因子：
56.9
作者：
BOYER, JS
通讯作者：
BOYER, JS
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作者：
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