Effect of leukemia inhibitory factor and forskolin on establishment of rat embryonic stem cell lines.
Effect of leukemia inhibitory factor and forskolin on establishment of rat embryonic stem cell lines.
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DOI：
10.1262/jrd.2013-109
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发表时间：
2014-03-07
期刊：
影响因子：
--
通讯作者：
Hochi S
中科院分区：
文献类型：
--
作者：
Hirabayashi M;Goto T;Tamura C;Sanbo M;Hara H;Hochi S
本研究旨在探讨在2i培养基中添加白血病抑制因子（LIF）和/或福斯克林是否有助于建立种系胜任型大鼠胚胎干（ES）细胞系。由于更高的生长率，添加或不添加LIF的forskolin有助于提高WDB菌株ES细胞系的建立效率。在胚胎干细胞系建立之前，嵌合大鼠的种系传播能力不受胚胎干细胞系结构的影响。当使用添加LIF/forskolin的2i培养基时，作为囊胚供体的大鼠菌株，如WI菌株，可能是影响ES细胞系建立效率的一个不利因素。一旦胚胎干细胞系被建立，通过嵌合大鼠的后代试验发现所有系都具有生殖系能力。综上所述，LIF和forskolin都不是必需的，但在“真正的”大鼠ES细胞系的建立中可以发挥有益的作用。
This study was designed to investigate whether supplementation of 2i medium with leukemia inhibitory factor (LIF) and/or forskolin would support establishment of germline-competent rat embryonic stem (ES) cell lines. Due to the higher likelihood of outgrowth rates, supplementation of forskolin with or without LIF contributed to the higher establishment efficiency of ES cell lines in the WDB strain. Germline transmission competency of the chimeric rats was not influenced by the profile of ES cell lines until their establishment. When the LIF/forskolin-supplemented 2i medium was used, the rat strain used as the blastocyst donor, such as the WI strain, was a possible factor negatively influencing the establishment efficiency of ES cell lines. Once ES cell lines were established, all lines were found to be germline-competent by a progeny test in chimeric rats. In conclusion, both LIF and forskolin are not essential but can play a beneficial role in the establishment of “genuine” rat ES cell lines.
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