Ti (WT31)-negative, CD3-positive, large granular lymphocyte leukemia with nonspecific cytotoxicity.
Ti (WT31)-negative, CD3-positive, large granular lymphocyte leukemia with nonspecific cytotoxicity.
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Ti (WT31) 阴性、CD3 阳性、大颗粒淋巴细胞白血病，具有非特异性细胞毒性。
DOI：
10.1182/blood.v71.4.923.bloodjournal714923
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发表时间：
1988
期刊：
影响因子：
20.3
通讯作者：
Y. Oshimi
中科院分区：
文献类型：
--
作者：
K. Oshimi;S. Hoshino;Masatomo Takahashi;M. Akahoshi;H. Saito;Y. Kobayashi;H. Hirai;F. Takaku;N. Yahagi;Y. Oshimi
报道一例WT31-、CD3+大颗粒淋巴细胞白血病。表面标记分析发现增殖细胞为CD3+4-8-16+和WT31-。通过双色免疫荧光染色，发现CD3+4-8-细胞为WT31-，少数WT31+细胞表达CD4或CD8。 WT31-、CD3+细胞也在体外扩增的淋巴细胞的大量培养物中被鉴定。由于 WT31 单克隆抗体 (MoAb) 与 T 细胞抗原受体 (Ti) 的二硫键连接的异二聚体的非多态性表位反应，因此 WT31 反应性 Ti 决定簇的缺失可能代表不同 CD3 相关多肽的表达。证明了 Ti-β 和 Ti-gamma 基因的重排，但免疫球蛋白基因没有重排，并且单一的重排模式表明淋巴细胞的单克隆起源。当检测淋巴细胞针对 K562、MOLT-4、Daudi 和 Raji 肿瘤细胞系的细胞毒性时，观察到对这些肿瘤细胞的广谱细胞毒性，并且淋巴细胞还表现出抗体和凝集素依赖性细胞毒性和淋巴因子激活的杀伤活性。用抗CD2和抗CD3 MoAb治疗抑制了它们的非特异性细胞毒性。抗CD3介导的非特异性细胞毒性抑制表明，与这些淋巴细胞上的CD3分子相关的尚未鉴定的Ti可作为靶肿瘤细胞识别的特异性受体。
A case of WT31-, CD3+ large granular lymphocyte leukemia is reported. On surface marker analysis, the proliferating cells were found to be CD3+4-8-16+ and WT31-. By two-color immunofluorescence staining, CD3+4-8- cells were found to be WT31-, and a small population of WT31+ cells expressed either CD4 or CD8. WT31-, CD3+ cells were also identified in a bulk culture of lymphocytes expanded in vitro. Because WT31 monoclonal antibody (MoAb) reacts with the nonpolymorphic epitope of the disulfide-linked heterodimer of the T cell antigen receptor (Ti), the absence of the WT31-reactive Ti determinant may represent an expression of different CD3-associated polypeptides. The rearrangement of the Ti-beta and Ti-gamma genes but not the immunoglobulin gene was demonstrated, and the single pattern of rearrangement indicated the monoclonal origin of the lymphocytes. When the lymphocytes were assayed for their cytotoxicity against K562, MOLT-4, Daudi, and Raji tumor cell lines, a broad spectrum of cytotoxicity for these tumor cells was observed, and the lymphocytes also exhibited antibody- and lectin-dependent cellular cytotoxicity and lymphokine-activated killer activity. Treatment with anti-CD2 and anti-CD3 MoAbs inhibited their nonspecific cytotoxicity. The anti-CD3-mediated inhibition of nonspecific cytotoxicity suggested that an as yet unidentified Ti, present in association with the CD3 molecule on these lymphocytes, serves as a specific receptor for target tumor cell recognition.
影响因子：
4.4
作者：
B. Perussia;G. Trinchieri;A. Jackson;N. Warner;J. Faust;H. Rumpold;D. Kraft;L. Lanier
通讯作者：
B. Perussia;G. Trinchieri;A. Jackson;N. Warner;J. Faust;H. Rumpold;D. Kraft;L. Lanier