Patterns of MADS-box gene expression mark flower-type development in Gerbera hybrida (Asteraceae).
Patterns of MADS-box gene expression mark flower-type development in Gerbera hybrida (Asteraceae).
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DOI：
10.1186/1471-2229-6-11
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发表时间：
2006-06-09
影响因子：
5.3
通讯作者：
Elomaa P
中科院分区：
文献类型：
--
作者：
Laitinen RA;Broholm S;Albert VA;Teeri TH;Elomaa P
切花作物非洲菊（菊科）的花序由两种主要的花型组成，即花丝和花盘，它们形成一个紧密排列的头状花序或头状花序。尽管对植物形态进化和非洲菊系统的易处理性有很大的兴趣，但对花型特化的遗传机制知之甚少。在这里，我们提供比较分期射线和盘花的发展和微阵列筛选差异表达的基因，通过显微解剖数百个协调发展的花原基。使用9 K非洲菊cDNA微阵列，我们确定了一些基因与推定的特异性个别花类型。有趣的是，其中几个编码同源的MADS盒转录因子，否则已知调节花器官发育。从这些和以前获得的数据，我们假设几个非洲菊MADS盒因子的功能和蛋白质-蛋白质相互作用。我们的RNA表达结果表明，花型特异性MADS蛋白复合物可能发挥核心作用，在整个非洲菊头状花序的射线和盘花的差异发展，并共享一些共性与已知的蛋白质功能在花器官的决定。这些发现支持了一个有趣的猜想，即非洲菊开花的头状花序不仅仅是基因调控水平上的花类似物。
The inflorescence of the cut-flower crop Gerbera hybrida (Asteraceae) consists of two principal flower types, ray and disc, which form a tightly packed head, or capitulum. Despite great interest in plant morphological evolution and the tractability of the gerbera system, very little is known regarding genetic mechanisms involved in flower type specification. Here, we provide comparative staging of ray and disc flower development and microarray screening for differentially expressed genes, accomplished via microdissection of hundreds of coordinately developing flower primordia. Using a 9K gerbera cDNA microarray we identified a number of genes with putative specificity to individual flower types. Intrestingly, several of these encode homologs of MADS-box transcription factors otherwise known to regulate flower organ development. From these and previously obtained data, we hypothesize the functions and protein-protein interactions of several gerbera MADS-box factors. Our RNA expression results suggest that flower-type specific MADS protein complexes may play a central role in differential development of ray and disc flowers across the gerbera capitulum, and that some commonality is shared with known protein functions in floral organ determination. These findings support the intriguing conjecture that the gerbera flowering head is more than a mere floral analog at the level of gene regulation.
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影响因子：
64.8
作者：
Doebley, J;Stec, A;Hubbard, L
通讯作者：
Hubbard, L
影响因子：
7.2
作者：
Elomaa, P;Mehto, M;Teeri, TH
通讯作者：
Teeri, TH
影响因子：
4.1
作者：
Greve, K;La Cour, T;Skriver, K
通讯作者：
Skriver, K
影响因子：
11.4
作者：
Egea-Cortines, M;Saedler, H;Sommer, H
通讯作者：
Sommer, H
影响因子：
11.6
作者：
Green, KA;Prigge, MJ;Clark, SE
通讯作者：
Clark, SE