Molecular cloning and characterization of endogenous feline leukemia virus sequences from a cat genomic library
Molecular cloning and characterization of endogenous feline leukemia virus sequences from a cat genomic library
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猫基因组文库中内源性猫白血病病毒序列的分子克隆和表征
DOI：
10.1128/jvi.46.3.829-840.1983
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发表时间：
1983
影响因子：
5.4
通讯作者：
Pradip ROY
中科院分区：
文献类型：
--
作者：
L. Soe;B. G. Devi;J. Mullins;Pradip ROY
从猫胎盘DNA基因组文库中筛选重组噬菌体lambda克隆，用于鉴定内源性猫白血病病毒(FeLV)序列。四个不同克隆的限制性内切酶图谱表明，它们之间有许多相似之处，特别是存在一个6.0-6.4kb碱基对(KBP)的EcoRI杂交片段，该片段包含与感染性FeLV的Gag、Pol1、env和长末端重复序列样元件同源的部分序列。分离到的FeLV内源序列长度约为4kbp，明显短于克隆的感染性FeLV分离株，后者的长度为8.5~8.7kbp。内源元件在gag-pol区有3.3-3.6kbp的缺失，在env区有大约0.7-1.0kbp的缺失。这些缺失将使它们不能编码感染性病毒，因此可能与未感染的猫细胞不能诱导FeLV以及这些内源性序列在胎盘组织中的亚基因组表达有关。这表明，在感染性FeLV的前病毒前体区中，与Pol1和env边界以及跨越内源克隆env基因的区域相对应的序列中，限制性内切酶切点的顺序似乎是保守的，而定位到Gag的限制性切点和感染性FeLV的部分Poll区域之间出现了更大的差异。这种缺失的、与FeLV相关的DNA序列亚集可能是通过完整的生态病毒的种系整合然后缺失，或者通过整合先前存在的、有缺陷的、缺失的传染性病毒变体而产生的。
Recombinant bacteriophage lambda clones from a cat genomic library derived from placental DNA of a specific pathogen-free cat were screened to identify endogenous feline leukemia virus (FeLV) sequences. Restriction endonuclease mapping of four different clones indicates that there are a number of similarities among them, notably the presence of a 6.0- to 6.4-kilobase pair (kbp) EcoRI hybridizing fragment containing portions of sequences homologous to the gag, pol, env, and long terminal repeat-like elements of the infectious FeLV. The endogenous FeLV sequences isolated are approximately 4 kbp in length and are significantly shorter than the cloned infectious FeLV isolates, which are 8.5 to 8.7 kbp in length. The endogenous elements have 3.3- to 3.6-kbp deletions in the gag-pol region and approximately 0.7- to 1.0-kbp deletions in the env region. These deletions would render them incapable of encoding an infectious virus and may therefore be related to the non-inducibility of FeLV from uninfected cat cells and the subgenomic expression of these endogenous sequences in placental tissue. It appears that there is conservation in the ordering of restriction sites previously reported in the proviruses of the infectious FeLVs in sequences corresponding to the pol and env boundary as well as the region spanning the env gene of the endogenous clones, whereas a greater divergence occurs among restriction sites mapped to the gag and part of the pol regions of the infectious FeLV. Such deleted, FeLV-related subsets of DNA sequences could have originated either by germ-line integration of a complete ecotropic virus followed by deletion, or by integration of a preexisting, defective, deleted variant of the infectious virus.
受感染细胞中猫白血病病毒 DNA 的序列组织。
影响因子：
14.9
作者：
Mullins,JI;Casey,JW;Nicolson,MO;Davidson,N
通讯作者：
Davidson,N