The Biocontrol Agent and Insect Pathogen Photorhabdus luminescens Interacts with Plant Roots
The Biocontrol Agent and Insect Pathogen Photorhabdus luminescens Interacts with Plant Roots
复制标题
生物防治剂和昆虫病原体发光杆菌与植物根部相互作用
DOI：
--
复制
发表时间：
2020
影响因子：
4.4
通讯作者：
R. Heermann
中科院分区：
文献类型：
--
作者：
Alice Regaiolo;N. Dominelli;Karsten Andresen;R. Heermann
昆虫致病性发光光habdus luminesens细菌被广泛应用于害虫的生物防治策略。人们对这些细菌在根际的生活知之甚少。在这里，我们发现P. luminescens可以特异性地与植物根系发生反应和相互作用。了解发光假单胞菌在根际的适应性对昆虫病原菌的生物技术应用和未来农业害虫的可持续治理具有重要意义。随着生物防治剂被用于增强作物的抗病性和非生物抗逆性，用于改善病虫害管理和环境安全的可持续农业技术的数量正在增加。本文研究了光habdus luminescens次生变异对植物根系分泌物的反应能力及其对根际微生物的行为。众所周知，P. luminescens与昆虫病原线虫（EPNs）共生，对昆虫具有高度致病性。发光紫霉与epn的关系已被广泛研究，该组合已被用作生物防治剂；然而，人们对其在根际的生活方式却不甚关注。我们进行了转录组分析，以显示P. luminescens对植物根系分泌物的反应。分析强调了涉及几丁质降解、生物膜调节、鞭毛形成和VI型分泌系统的基因。此外，我们提供的证据表明，P. luminescens可以抑制植物病原真菌的生长。最后，我们证明了发光假单胞菌与植物根系的特定相互作用。了解这种细菌在根际的作用和功能，有助于促进生物防治的进展，并阐明植物生长促进菌在植物根系相互作用中所采取的特殊机制。昆虫致病性发光光habdus luminesens细菌被广泛应用于害虫的生物防治策略。人们对这些细菌在根际的生活知之甚少。在这里，我们发现P. luminescens可以特异性地与植物根系发生反应和相互作用。了解发光假单胞菌在根际的适应性对昆虫病原菌的生物技术应用和未来农业害虫的可持续治理具有重要意义。
Insect-pathogenic Photorhabdus luminescens bacteria are widely used in biocontrol strategies against pests. Very little is known about the life of these bacteria in the rhizosphere. Here, we show that P. luminescens can specifically react to and interact with plant roots. Understanding the adaptation of P. luminescens in the rhizosphere is highly important for the biotechnological application of entomopathogenic bacteria and could improve future sustainable pest management in agriculture. ABSTRACT The number of sustainable agriculture techniques to improve pest management and environmental safety is rising, as biological control agents are used to enhance disease resistance and abiotic stress tolerance in crops. Here, we investigated the capacity of the Photorhabdus luminescens secondary variant to react to plant root exudates and their behavior toward microorganisms in the rhizosphere. P. luminescens is known to live in symbiosis with entomopathogenic nematodes (EPNs) and to be highly pathogenic toward insects. The P. luminescens-EPN relationship has been widely studied, and this combination has been used as a biological control agent; however, not much attention has been paid to the putative lifestyle of P. luminescens in the rhizosphere. We performed transcriptome analysis to show how P. luminescens responds to plant root exudates. The analysis highlighted genes involved in chitin degradation, biofilm regulation, formation of flagella, and type VI secretion system. Furthermore, we provide evidence that P. luminescens can inhibit growth of phytopathogenic fungi. Finally, we demonstrated a specific interaction of P. luminescens with plant roots. Understanding the role and the function of this bacterium in the rhizosphere might accelerate the progress in biocontrol manipulation and elucidate the peculiar mechanisms adopted by plant growth-promoting rhizobacteria in plant root interactions. IMPORTANCE Insect-pathogenic Photorhabdus luminescens bacteria are widely used in biocontrol strategies against pests. Very little is known about the life of these bacteria in the rhizosphere. Here, we show that P. luminescens can specifically react to and interact with plant roots. Understanding the adaptation of P. luminescens in the rhizosphere is highly important for the biotechnological application of entomopathogenic bacteria and could improve future sustainable pest management in agriculture.
DOI：
10.1038/ismej.2016.169
发表时间：
2017-04
期刊：
The ISME journal
影响因子：
--
作者：
Bernal P;Allsopp LP;Filloux A;Llamas MA
通讯作者：
Llamas MA
影响因子：
5.4
作者：
Shank, Elizabeth Anne;Kolter, Roberto
通讯作者：
Kolter, Roberto
影响因子：
30.3
作者：
Ho BT;Dong TG;Mekalanos JJ
通讯作者：
Mekalanos JJ