Sensitive intranuclear flow cytometric quantification of IRF4 protein in multiple myeloma and normal human hematopoietic cells.
Sensitive intranuclear flow cytometric quantification of IRF4 protein in multiple myeloma and normal human hematopoietic cells.
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DOI：
10.1016/j.xpro.2021.100565
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发表时间：
2021-06-18
期刊：
影响因子：
--
通讯作者：
Crews LA
中科院分区：
文献类型：
--
作者：
Vora AA;Mondala PK;Costello C;MacLeod AR;Crews LA
Interferon regulatory factor 4 (IRF4) is a transcription factor that regulates normal and malignant immune cell development and is implicated in multiple myeloma pathogenesis. This protocol describes the use of combined cell surface and intranuclear staining with fluorescent antibodies to measure IRF4 protein expression within myeloma and normal immune cells. IRF4 protein quantification may provide a valuable prognostic tool to predict disease severity and sensitivity to IRF4-targeted therapies. This flow-cytometry-based procedure could also be rapidly translated into a clinically compatible assay. For complete details on the use and execution of this protocol, please refer to. Protocol for the quantification of human IRF4 levels by multi-parameter flow cytometry Facilitates sensitive detection of IRF4 protein in myeloma cells and normal bone marrow High-risk myeloma is typified by a population of CD38-high, IRF4-enriched cells Interferon regulatory factor 4 (IRF4) is a transcription factor that regulates normal and malignant immune cell development and is implicated in multiple myeloma pathogenesis. This protocol describes the use of combined cell surface and intranuclear staining with fluorescent antibodies to measure IRF4 protein expression within myeloma and normal immune cells. IRF4 protein quantification may provide a valuable prognostic tool to predict disease severity and sensitivity to IRF4-targeted therapies. This flow-cytometry-based procedure could also be rapidly translated into a clinically compatible assay.
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影响因子：
64.8
作者：
Shaffer, Arthur L.;Emre, N. C. Tolga;Staudt, Louis M.
通讯作者：
Staudt, Louis M.
影响因子：
7.4
作者：
Crews LA;Jiang Q;Zipeto MA;Lazzari E;Court AC;Ali S;Barrett CL;Frazer KA;Jamieson CH
通讯作者：
Jamieson CH
影响因子：
23.9
作者：
Crews, Leslie A.;Balaian, Larisa;Delos Santos, Nathaniel P.;Leu, Heather S.;Court, Angela C.;Lazzari, Elisa;Sadarangani, Anil;Zipeto, Maria A.;La Clair, James J.;Villa, Reymundo;Kulidjian, Anna;Storb, Rainer;Morris, Sheldon R.;Ball, Edward D.;Burkart, Michael D.;Jamieson, Catriona H. M.
通讯作者：
Jamieson, Catriona H. M.
影响因子：
8.8
作者：
Jiang Q;Isquith J;Ladel L;Mark A;Holm F;Mason C;He Y;Mondala P;Oliver I;Pham J;Ma W;Reynoso E;Ali S;Morris IJ;Diep R;Nasamran C;Xu G;Sasik R;Rosenthal SB;Birmingham A;Coso S;Pineda G;Crews L;Donohoe ME;Venter JC;Whisenant T;Mesa RA;Alexandrov LB;Fisch KM;Jamieson C
通讯作者：
Jamieson C
影响因子：
6.5
作者：
Lopez-Girona, Antonia;Heintel, Daniel;Chopra, Rajesh
通讯作者：
Chopra, Rajesh