Serum or Growth Factor Deprivation Induces the Expression of Alkaline Phosphatase in Human Gingival Fibroblasts
Serum or Growth Factor Deprivation Induces the Expression of Alkaline Phosphatase in Human Gingival Fibroblasts
复制标题
血清或生长因子剥夺诱导人牙龈成纤维细胞中碱性磷酸酶的表达
DOI：
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发表时间：
1998
期刊：
影响因子：
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通讯作者：
K. Maeda
中科院分区：
文献类型：
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作者：
T. Abe;Y. Hara;Y. Abe;Y. Aida;K. Maeda
We have previously reported that the increased expression of alkaline phosphatase (ALP) activity is a phenotypic characteristic of gingival fibroblasts present in chronic inflammatory periodontal lesions. We hypothesized that ALP might be induced in gingival fibroblasts by environmental factors. In the present study, we investigated the factors influencing the induction of ALP expression in fibroblasts derived from healthy human gingiva. The withdrawal of serum from confluent cultures of fibroblasts increased the number of cells positive for ALP activity and protein, without their proliferation. Suramin, a growth factor antagonist, induced ALP expression in cells cultured with serum. Serum re-addition or exposure to platelet-derived growth factor-AB and/or insulin-like growth factor I suppressed ALP induction and caused cell growth. ALP-positive cells could survive for up to 6 weeks after serum deprivation, a condition inducing cell death via apoptosis. These results demonstrate that serum or growth factor deprivation induces the expression of ALP in gingival fibroblasts. ALP expression is negatively correlated with cell growth and accompanied by a change into serum-growth-factor-independent survival.
酸性和碱性成纤维细胞生长因子是在静止的 BALB/c 3T3 小鼠成纤维细胞中具有独特活性的存活因子。
DOI：
10.1073/pnas.88.8.3372
发表时间：
1991
影响因子：
11.1
作者：
Tamm,I;Kikuchi,T;Zychlinsky,A
通讯作者：
Zychlinsky,A
30 kDa 末端蛋白的出现标志着细胞死亡的发生。
影响因子：
3.7
作者：
Hebert,L;Pandey,S;Wang,E
通讯作者：
Wang,E