Seroepidemiology of pandemic influenza A (H1N1) 2009 virus infections in Pune, India.
Seroepidemiology of pandemic influenza A (H1N1) 2009 virus infections in Pune, India.
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DOI：
10.1186/1471-2334-10-255
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发表时间：
2010-08-25
影响因子：
3.7
通讯作者：
Mishra AC
中科院分区：
文献类型：
--
作者：
Tandale BV;Pawar SD;Gurav YK;Chadha MS;Koratkar SS;Shelke VN;Mishra AC
在印度，浦那是2009年甲型H1N1流感大流行期间受影响最严重的城市之一。我们对危险人群和普通人群进行了血清调查，以确定2009年甲型H1N1流感大流行病毒感染的程度。对2005年1月至2009年3月期间从档案中收集的大流行前血清进行了分析，以确定基线血清阳性。在2009年8月15日至12月11日期间，对医院工作人员、全科医生、学童和工作人员以及一般人群等风险群体进行了血清调查。此外，还对经pcr确诊的2009年甲型H1N1大流行性流感病例及其家庭接触者进行了调查。血凝抑制（HI）试验采用标准方案使用火鸡红细胞进行。滴度≥1:40为血清阳性。222份大流行前血清中只有2份（0.9%）呈阳性。流感大流行H1N1、季节性H1N1和季节性H3N2的重测信度分别为98%、93.1%和94%。2009年65例聚合酶链反应确诊的H1N1大流行病例中48例（73.8%）血清呈阳性。全科医生血清阳性从8月的4.9%上升到11月的9.4%和12月的15.1%。在医院工作人员中，血清阳性从8月份的2.8%上升到11月份的12%。学校的血清阳性率由8月的2%上升至9月的10.7%。9月学生血清阳性率（25%）高于学校教职员。在2009年10月的一次普通人群调查中，儿童（9.1%）的血清阳性率高于成人（4.3%）。15 ~ 19岁年龄组血清阳性率最高，为20.3%。季节性H3N2（55.3%）和H1N1（26.4%）血清阳性率高于大流行H1N1 (5.7%) （n = 2328）。在74例聚合酶链反应确诊的H1N1大流行病例的家庭中，25.6%的接触者血清呈阳性。几乎90%的H1N1大流行感染是无症状或轻微的。考虑到滴度切断为1:10，血清阳性是1:40的1.5-3倍。2009年甲型H1N1流感大流行性病毒感染在社区各阶层普遍存在。然而，在校儿童和全科医生的感染率明显较高。医院工作人员的感染率最低，表明感染控制措施的有效性。
In India, Pune was one of the badly affected cities during the influenza A (H1N1) 2009 pandemic. We undertook serosurveys among the risk groups and general population to determine the extent of pandemic influenza A (H1N1) 2009 virus infections. Pre-pandemic sera from the archives, collected during January 2005 to March 2009, were assayed for the determination of baseline seropositivity. Serosurveys were undertaken among the risk groups such as hospital staff, general practitioners, school children and staff and general population between 15th August and 11th December 2009. In addition, the PCR-confirmed pandemic influenza A (H1N1) 2009 cases and their household contacts were also investigated. Haemagglutination-inhibition (HI) assays were performed using turkey red blood cells employing standard protocols. A titre of ≥1:40 was considered seropositive. Only 2 (0.9%) of the 222 pre-pandemic sera were positive. The test-retest reliability of HI assay in 101 sera was 98% for pandemic H1N1, 93.1% for seasonal H1N1 and 94% for seasonal H3N2. The sera from 48 (73.8%) of 65 PCR-confirmed pandemic H1N1 cases in 2009 were positive. Seropositivity among general practitioners increased from 4.9% in August to 9.4% in November and 15.1% in December. Among hospital staff, seropositivity increased from 2.8% in August to 12% in November. Seropositivity among the schools increased from 2% in August to 10.7% in September. The seropositivity among students (25%) was higher than the school staff in September. In a general population survey in October 2009, seropositivity was higher in children (9.1%) than adults (4.3%). The 15-19 years age group showed the highest seropositivity of 20.3%. Seropositivity of seasonal H3N2 (55.3%) and H1N1 (26.4%) was higher than pandemic H1N1 (5.7%) (n = 2328). In households of 74 PCR-confirmed pandemic H1N1 cases, 25.6% contacts were seropositive. Almost 90% pandemic H1N1 infections were asymptomatic or mild. Considering a titre cut off of 1:10, seropositivity was 1.5-3 times as compared to 1:40. Pandemic influenza A (H1N1) 2009 virus infection was widespread in all sections of community. However, infection was significantly higher in school children and general practitioners. Hospital staff had the lowest infections suggesting the efficacy of infection-control measures.
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DOI：
10.1056/nejmoa0905498
发表时间：
2009-12-31
期刊：
The New England journal of medicine
影响因子：
--
作者：
Cauchemez S;Donnelly CA;Reed C;Ghani AC;Fraser C;Kent CK;Finelli L;Ferguson NM
通讯作者：
Ferguson NM
影响因子：
3
作者：
Chan, Yu-Jiun;Lee, Chia-Ling;Lee, Shou-Dong
通讯作者：
Lee, Shou-Dong
影响因子：
5.4
作者：
Allwinn, Regina;Geiler, Janina;Doerr, H. W.
通讯作者：
Doerr, H. W.
影响因子：
3.7
作者：
Lee VJ;Yap J;Tay JK;Barr I;Gao Q;Ho HJ;Tan BH;Kelly PM;Tambyah PA;Kelso A;Chen MI
通讯作者：
Chen MI
DOI：
10.1056/nejmp0904380
发表时间：
2009-07-09
期刊：
The New England journal of medicine
影响因子：
--
作者：
Lipsitch M;Riley S;Cauchemez S;Ghani AC;Ferguson NM
通讯作者：
Ferguson NM