Commensal bacteria-dependent indole production enhances epithelial barrier function in the colon.
Commensal bacteria-dependent indole production enhances epithelial barrier function in the colon.
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DOI：
10.1371/journal.pone.0080604
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Takeda K
中科院分区：
文献类型：
--
作者：
Shimada Y;Kinoshita M;Harada K;Mizutani M;Masahata K;Kayama H;Takeda K
微生物群对肠上皮细胞（ECs）的功能有很大的影响。吲哚作为群体感应（QS）分子在细菌中介导细胞间信号的作用已经得到了很好的认识。然而，吲哚是否对维持体内肠道屏障有有益作用尚不清楚。本研究采用无菌小鼠模型，分析吲哚对内皮细胞的影响。GF小鼠结肠内皮细胞中连接复合物分子表达降低。SPF （specific pathogen-free）小鼠粪便中含有大量吲哚；而在GF小鼠的粪便中，其含量明显减少了27倍。口服含吲哚胶囊可增加GF小鼠结肠内皮细胞中紧密连接(TJ)-和粘附连接(AJ)-相关分子的表达。根据这些连接复合物分子表达的增加，给予含吲哚胶囊的GF小鼠对葡聚糖硫酸钠（DSS）诱导的结肠炎表现出更高的抵抗力。在SPF条件下饲养的小鼠中也观察到吲哚对dss诱导的上皮损伤的类似保护作用。这些发现强调了吲哚在体内建立上皮屏障中的有益作用。
Microbiota have been shown to have a great influence on functions of intestinal epithelial cells (ECs). The role of indole as a quorum-sensing (QS) molecule mediating intercellular signals in bacteria has been well appreciated. However, it remains unknown whether indole has beneficial effects on maintaining intestinal barriers in vivo. In this study, we analyzed the effect of indole on ECs using a germ free (GF) mouse model. GF mice showed decreased expression of junctional complex molecules in colonic ECs. The feces of specific pathogen-free (SPF) mice contained a high amount of indole; however the amount was significantly decreased in the feces of GF mice by 27-fold. Oral administration of indole-containing capsules resulted in increased expression of both tight junction (TJ)- and adherens junction (AJ)-associated molecules in colonic ECs in GF mice. In accordance with the increased expression of these junctional complex molecules, GF mice given indole-containing capsules showed higher resistance to dextran sodium sulfate (DSS)-induced colitis. A similar protective effect of indole on DSS-induced epithelial damage was also observed in mice bred in SPF conditions. These findings highlight the beneficial role of indole in establishing an epithelial barrier in vivo.
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影响因子：
5.7
作者：
Lee J;Attila C;Cirillo SL;Cirillo JD;Wood TK
通讯作者：
Wood TK
影响因子：
64.8
作者：
Fukuda, Shinji;Toh, Hidehiro;Ohno, Hiroshi
通讯作者：
Ohno, Hiroshi
DOI：
10.1007/bf03190428
发表时间：
2002-04-01
影响因子：
1.9
作者：
Banoglu, E;King, RS
通讯作者：
King, RS
影响因子：
4.2
作者：
Li, Nan;Neu, Josef
通讯作者：
Neu, Josef
影响因子：
1.6
作者：
Konishi, Y
通讯作者：
Konishi, Y