The metalloreductase FreB is involved in adaptation of Aspergillus fumigatus to iron starvation.
The metalloreductase FreB is involved in adaptation of Aspergillus fumigatus to iron starvation.
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DOI：
10.1016/j.fgb.2011.07.009
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发表时间：
2011-11
影响因子：
3
通讯作者：
Haas, Hubertus
中科院分区：
文献类型：
--
作者：
Blatzer, Michael;Binder, Ulrike;Haas, Hubertus
► 主要铁还原酶编码基因 FreB 的表征。 ► FreB 在适应铁饥饿方面的关键作用。 ► 铁和铜代谢之间的联系。 ► 铁载体系统增强了对铜缺乏的抵抗力。烟曲霉采用两种高亲和力铁吸收机制：铁载体介导的铁吸收和还原性铁同化（RIA）。烟曲霉基因组编码 15 种假定的金属还原酶 (MR)，但迄今为止，与 RIA 相关的铁还原酶仍然难以捉摸。发现 MR FreB 的表达通过 SreA 受到铁的转录抑制，SreA 是铁充足期间铁获取的抑制因子，表明在铁代谢中发挥作用。基因缺失导致的 FreB 失活在表型上基本上不明显，除非与铁载体系统失活相结合，从而降低生长速率、表面铁还原酶活性和铁饥饿期间的氧化应激抵抗力。这项研究还表明，由于RIA对铜的依赖性，烟曲霉对铜饥饿的敏感性增加，因此，铜不依赖的铁载体介导的铁吸收的丧失会增加。
► Characterization of a major ferrireductase encoding gene, FreB. ► Crucial role of FreB in adaptation to iron starvation. ► Link between iron and copper metabolism. ► Siderophore system increases resistance against copper starvation. Aspergillus fumigatus employs two high affinity iron uptake mechanisms, siderophore mediated iron uptake and reductive iron assimilation (RIA). The A. fumigatus genome encodes 15 putative metalloreductases (MR) but the ferrireductases involved in RIA remained elusive so far. Expression of the MR FreB was found to be transcriptionally repressed by iron via SreA, a repressor of iron acquisition during iron sufficiency, indicating a role in iron metabolism. FreB-inactivation by gene deletion was phenotypically largely inconspicuous unless combined with inactivation of the siderophore system, which then decreased growth rate, surface ferrireductase activity and oxidative stress resistance during iron starvation. This study also revealed that loss of copper-independent siderophore-mediated iron uptake increases sensitivity of A. fumigatus to copper starvation due to copper-dependence of RIA.
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