Ion mobility mass spectrometry analysis of isomeric disaccharide precursor, product and cluster ions.
Ion mobility mass spectrometry analysis of isomeric disaccharide precursor, product and cluster ions.
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DOI：
10.1002/rcm.6720
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发表时间：
2013-12-15
影响因子：
2
通讯作者：
Hill, Herbert H., Jr.
中科院分区：
文献类型：
--
作者：
Li, Hongli;Bendiak, Brad;Siems, William F.;Gang, David R.;Hill, Herbert H., Jr.
碳水化合物的结构变化很大，这是由于它们的异构体构型、单体立体化学、残基间连接位置和一般分支特征的不同。碳水化合物异构体的分离对目前的分析技术提出了巨大的挑战。用电喷雾行波离子迁移率质谱仪(Synapt G2高清晰度质谱仪)研究了正、负离子模式下双糖离子和单糖乙醛产物离子的异构性。根据异构体二糖离子的迁移率分布，观察到了异构体二糖离子的分离，但没有完全实现。测定了不同二糖异构体衍生的单糖-乙醛的异构化产物离子的迁移率。观察到单糖-乙醇醛阳离子和阴离子的多个迁移率峰，表明溶液中的核磁共振证实了气相中存在不止一种结构构型。更重要的是，异构体单糖-乙醇醛产物离子的迁移性模式不同，这使得它们能够部分表征它们各自的双糖离子。还观察到了丰富的双糖团簇离子。结果表明，大多数同分异构团簇离子具有不同的漂移时间，并且对一些特定的团簇离子检测到多个迁移峰。结果表明，离子迁移率质谱仪是评价碳水化合物异构体非均质性的一种有利方法。它能够区分具有相同m/z值的不同类型的碳水化合物离子以及单一化合物的多种结构构型。
Carbohydrates are highly variable in structure owing to differences in their anomeric configurations, monomer stereochemistry, inter-residue linkage positions and general branching features. The separation of carbohydrate isomers poses a great challenge for current analytical techniques. The isomeric heterogeneity of disaccharide ions and monosaccharideglycolaldehyde product ions evaluated using electrospray traveling wave ion mobility mass spectrometry (Synapt G2 high definition mass spectrometer) in both positive and negative ion modes investigation. The separation of isomeric disaccharide ions was observed but not fully achieved based on their mobility profiles. The mobilities of isomeric product ions, the monosaccharide-glycolaldehydes, derived from different disaccharide isomers were measured. Multiple mobility peaks were observed for both monosaccharide-glycolaldehyde cations and anions, indicating that there was more than one structural configuration in the gas phase as verified by NMR in solution. More importantly, the mobility patterns for isomeric monosaccharide-glycolaldehyde product ions were different, which enabled partial characterization of their respective disaccharide ions. Abundant disaccharide cluster ions were also observed. The Results showed that a majority of isomeric cluster ions had different drift times and, moreover, more than one mobility peak was detected for a number of specific cluster ions. It is demonstrated that ion mobility mass spectrometry is an advantageous method to assess the isomeric heterogeneity of carbohydrate compounds. It is capable of differentiating different types of carbohydrate ions having identical m/z values as well as multiple structural configurations of single compounds.
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影响因子：
3.3
作者：
Fenn, Larissa S.;McLean, John A.
通讯作者：
McLean, John A.
影响因子：
7.4
作者：
Kaplan, Kimberly;Dwivedi, Prabha;Hill, Herbert H., Jr.
通讯作者：
Hill, Herbert H., Jr.
影响因子：
15
作者：
KIM, MJ;HENNEN, WJ;WONG, CH
通讯作者：
WONG, CH
DOI：
10.1016/j.jasms.2007.04.007
发表时间：
2007-07-01
影响因子：
3.2
作者：
Dwivedi, Prabha;Bendiak, Brad;Hill, Herbert H., Jr.
通讯作者：
Hill, Herbert H., Jr.
影响因子：
3.1
作者：
Fang, Tammy T.;Zirrolli, Joseph;Bendiak, Brad
通讯作者：
Bendiak, Brad