Single-molecule sequencing to track plasmid diversity of hospital-associated carbapenemase-producing Enterobacteriaceae.
Single-molecule sequencing to track plasmid diversity of hospital-associated carbapenemase-producing Enterobacteriaceae.
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DOI：
10.1126/scitranslmed.3009845
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发表时间：
2014-09-17
影响因子：
17.1
通讯作者：
Segre JA
中科院分区：
文献类型：
--
作者：
Conlan S;Thomas PJ;Deming C;Park M;Lau AF;Dekker JP;Snitkin ES;Clark TA;Luong K;Song Y;Tsai YC;Boitano M;Dayal J;Brooks SY;Schmidt B;Young AC;Thomas JW;Bouffard GG;Blakesley RW;NISC Comparative Sequencing Program;Mullikin JC;Korlach J;Henderson DK;Frank KM;Palmore TN;Segre JA
公共卫生官员担心，肠杆菌科物种之间的质粒转移可能会传播对碳青霉烯类抗生素的耐药性，从而使常见的医疗保健相关感染几乎无法治疗。我们进行了全面的监测和基因组测序，以鉴定NIH临床中心患者群体和医院环境中的碳青霉烯耐药肠杆菌科细菌，以阐明碳青霉烯酶编码质粒的多样性，并调查和评估这些质粒在细菌物种之间的迁移率。我们分离到了一批编码碳青霉烯酶的肠杆菌科细菌，包括肺炎克雷伯菌、催产克雷伯氏菌、大肠杆菌、阴沟肠杆菌、弗劳地柠檬酸杆菌和泛亚杆菌等多种菌株。完整端到端组装的长时间基因组测序显示，这些生物携带碳青霉烯类抗生素耐药基因在广泛的一系列质粒上。同时从一名患者体内分离的肺炎克雷伯菌和阴沟肠杆菌含有两种不同的碳青霉烯酶编码质粒，推翻了该患者体内生物之间转移质粒的流行病学情景。然而，我们确实发现了支持医院环境中肺炎克雷伯菌、阴沟肠杆菌和弗劳地柠檬酸杆菌之间碳青霉烯酶编码质粒水平转移的证据。我们全面的序列数据，加上完整的质粒鉴定，挑战了关于患者体内水平基因转移事件的假设，并确定了患者与医院环境之间更广泛的可能联系。此外，我们从无关患者和医院环境中鉴定了肺炎克雷伯菌、大肠埃希菌、阴沟肠杆菌和泛亚单位携带的具有潜在高临床影响的新的碳青霉烯酶编码质粒。
Public health officials have raised concerns that plasmid transfer between Enterobacteriaceae species may spread resistance to carbapenems, an antibiotic class of last resort, thereby rendering common healthcare-associated infections nearly impossible to treat. We performed comprehensive surveillance and genomic sequencing to identify carbapenem-resistant Enterobacteriaceae in the NIH Clinical Center patient population and hospital environment in order to to articulate the diversity of carbapenemase-encoding plasmids and survey the mobility of and assess the mobility of these plasmids between bacterial species. We isolated a repertoire of carbapenemase-encoding Enterobacteriaceae, including multiple strains of Klebsiella pneumoniae, Klebsiella oxytoca, Escherichia coli, Enterobacter cloacae, Citrobacter freundii, and Pantoea species. Long-read genome sequencing with full end-to-end assembly revealed that these organisms carry the carbapenem-resistance genes on a wide array of plasmids. Klebsiella pneumoniae and Enterobacter cloacae isolated simultaneously from a single patient harbored two different carbapenemase-encoding plasmids, overriding the epidemiological scenario of plasmid transfer between organisms within this patient. We did, however, find evidence supporting horizontal transfer of carbapenemase-encoding plasmids between Klebsiella pneumoniae, Enterobacter cloacae and Citrobacter freundii in the hospital environment. Our comprehensive sequence data, with full plasmid identification, challenges assumptions about horizontal gene transfer events within patients and identified wider possible connections between patients and the hospital environment. In addition, we identified a new carbapenemase-encoding plasmid of potentially high clinical impact carried by Klebsiella pneumoniae, Escherichia coli, Enterobacter cloacae and Pantoea species, from unrelated patients and the hospital environment.
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影响因子：
4.9
作者：
Leavitt, Azita;Chmelnitsky, Inna;Navon-Venezia, Shiri
通讯作者：
Navon-Venezia, Shiri
影响因子：
9.4
作者：
Hindiyeh, Musa;Smollen, Gill;Keller, Nathan
通讯作者：
Keller, Nathan
与受污染的洗手水槽相关的产生延长的谱系β-内酰胺酶的β-内酰胺酶的爆发（1）。
影响因子：
11.8
作者：
Lowe C;Willey B;O'Shaughnessy A;Lee W;Lum M;Pike K;Larocque C;Dedier H;Dales L;Moore C;McGeer A;Mount Sinai Hospital Infection Control Team
通讯作者：
Mount Sinai Hospital Infection Control Team
DOI：
10.1126/science.1182395
发表时间：
2010-01-22
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Harris SR;Feil EJ;Holden MT;Quail MA;Nickerson EK;Chantratita N;Gardete S;Tavares A;Day N;Lindsay JA;Edgeworth JD;de Lencastre H;Parkhill J;Peacock SJ;Bentley SD
通讯作者：
Bentley SD
影响因子：
5.4
作者：
Hatoum-Aslan, Asma;Marraffini, Luciano A.
通讯作者：
Marraffini, Luciano A.