NMDA receptor NR1 and NR2A/B subunit expression in trigeminal neurons during early postnatal development
NMDA receptor NR1 and NR2A/B subunit expression in trigeminal neurons during early postnatal development
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出生后早期发育过程中三叉神经元 NMDA 受体 NR1 和 NR2A/B 亚基的表达
DOI：
10.1002/(sici)1096-9861(19990628)409:2
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发表时间：
1999
影响因子：
2.5
通讯作者：
S. H. Chandler
中科院分区：
文献类型：
--
作者：
J. Turman;Jurak Ajdari;S. H. Chandler
三叉神经运动神经元（Mo 5），中脑三叉神经神经元（Me 5），三叉神经上（Su 5）和三叉神经间（I5）神经元的神经回路的重要组成部分，负责在摄食行为期间观察到的下颌运动。此外，在成年动物中，N-甲基-D-天冬氨酸（NMDA）受体是脑干回路的关键组成部分，负责反射和中枢激活的下颌运动。然而，很少有人知道这种受体的表达在电路用于产生新生儿下颌运动。用受体免疫组织化学方法描述了NMDA NR 1和NR 2 A/B受体亚单位在Mo 5、Me 5、Su 5和I5神经元中的表达在生后发育过程中的变化。使用出生后（P）1、3、8、15-16、21-24和28-35天的大鼠。使用针对NR 1亚基的亲和纯化的多克隆抗体和识别NR 2A和2B亚基的亲和纯化的多克隆抗体来描述这些亚基的表达。在Mo 5中，在整个采样时间范围内观察到两种抗体的免疫反应性。NR 1在Me 5神经元的表达出现在P1。NR 2 A/B在P3出现于Me 5核的尾侧和中部区域，在P8出现于核的吻侧区域。NR 1免疫反应性存在于P1的神经元在I5和P3的神经元在Su 5区。NR 2A/B亚单位在P8时出现于Su 5和I5神经元。这些结果提供了证据，NMDA受体亚单位在新生儿三叉神经元用于口腔运动电路，并建议NMDA受体在突触发生与这些神经元在出生后的发展过程中的作用。神经学比较杂志409：237-249，1999.© 1999 Wiley利斯公司
Trigeminal motoneurons (Mo5), mesencephalic trigeminal neurons (Me5), and supratrigeminal (Su5) and intertrigeminal (I5) neurons are important constituents of the neural circuitry responsible for jaw movements observed during ingestive behaviors. In addition, in adult animals, N‐methyl‐D‐aspartate (NMDA) receptors are a critical component of the brainstem circuitry responsible for reflex‐ and centrally activated jaw movements. However, little is known about the expression of this receptor in circuitry used to produce neonatal jaw movements. Receptor immunohistochemistry was used to describe changes in the expression of NMDA NR1 and NR2A/B receptor subunits in Mo5, Me5, Su5, and I5 neurons during postnatal development. Rats at postnatal days (P) 1, 3, 8, 15–16, 21–24, and 28–35 were used. An affinity‐purified polyclonal antibody against the NR1 subunit and an affinity‐purified polyclonal antibody that recognizes both NR2A and 2B subunits were used to depict the expression of these subunits. In Mo5, immunoreactivity was noted for both antibodies throughout the time frame sampled. NR1 expression in Me5 neurons emerged at P1. NR2A/B expression emerged at P3 in caudal and middle regions of Me5 and at P8 for rostral regions of the nucleus. NR1 immunoreactivity was present at P1 for neurons in I5 and at P3 for neurons in the Su5 region. NR2A/B subunit expression in Su5 and I5 neurons emerged at P8. These results provide evidence for NMDA receptor subunits in neonatal trigeminal neurons used in oral–motor circuitry and suggest a role for the NMDA receptor in synaptogenesis associated with these neurons during postnatal development. J. Comp. Neurol. 409:237–249, 1999. © 1999 Wiley‐Liss, Inc.
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影响因子：
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作者：
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DOI：
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期刊：
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影响因子：
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