A novel biomarker harvesting nanotechnology identifies Bak as a candidate melanoma biomarker in serum.
A novel biomarker harvesting nanotechnology identifies Bak as a candidate melanoma biomarker in serum.
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DOI：
10.1111/j.1600-0625.2010.01187.x
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发表时间：
2011-01
影响因子：
3.6
通讯作者：
Liotta LA
中科院分区：
文献类型：
--
作者：
Longo C;Gambara G;Espina V;Luchini A;Bishop B;Patanarut AS;Petricoin EF 3rd;Beretti F;Ferrari B;Garaci E;De Pol A;Pellacani G;Liotta LA
黑色素瘤仅占所有皮肤癌的4%，但占皮肤癌死亡人数的近80%。这份手稿应用了几种新的测量技术，目的是阐明黑色素瘤侵袭性的分子特征。我们试图确定是否可以测量与凋亡途径相关的低丰度血清蛋白，并与定义的黑色素瘤亚型相关。水凝胶核壳纳米颗粒是一种能够选择性地捕获低分子量蛋白质并保护它们免受酶降解的新技术，用于捕获候选血清生物标志物。生物标志物水平与共聚焦显微镜相关，因此代表了体内组织学记录的新技术组合。在一组分析的血清蛋白中，巴克在痣和黑色素瘤之间差异表达。具有较高巴克血清水平的黑素瘤在共聚焦成像上表现出更明显的连接活性，而具有“稀疏”真皮巢的病变具有较弱的巴克表达。我们的研究将血清蛋白质组分析与黑色素瘤的共聚焦显微镜临床组织学分类联系起来。之前未在血清中测量过巴克。黑色素瘤亚型之间的巴克差异表达证实了细胞凋亡途径作为黑色素瘤侵袭性的贡献者的重要性。
Melanoma represents only 4% of all skin cancers, but nearly 80% of skin cancer deaths. This manuscript applies several new measurement technologies with the purpose of elucidating molecular signatures of melanoma aggressiveness. We sought to determine whether low-abundant serum proteins related to apoptotic pathways could be measured and correlated with defined melanoma subtypes. Hydrogel core shell nanoparticles, a new technology capable of selectively entrapping low molecular weight proteins and protecting them from enzymatic degradation, were used to capture candidate serum biomarkers. Biomarker levels were correlated with confocal microscopy, thereby representing a combination of new technologies for in vivo histologic documentation. Among a panel of analyzed serum proteins, Bak was differentially expressed between nevi and melanomas. Melanomas with higher Bak serum levels exhibited more pronounced junctional activity on confocal imaging, whereas lesions with ‘sparse’ dermal nests had weak Bak expression. Our study links serum proteome analysis with confocal microscopic clinical in vivo histologic classification of melanomas. Bak has not been previously measured in serum. Bak differential expression among melanoma subtypes confirms the importance of the apoptotic pathway as a contributor to melanoma aggressiveness.
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影响因子：
6.5
作者：
Fecker, Lothar F.;Geilen, Christoph C.;Eberle, Juergen
通讯作者：
Eberle, Juergen
影响因子：
--
作者：
Pellacani, G;Cesinaro, AM;Seidenari, S
通讯作者：
Seidenari, S
影响因子：
10.3
作者：
Barnhill, RL;Xiao, M;Antoniades, HN
通讯作者：
Antoniades, HN
影响因子：
--
作者：
Luchini A;Longo C;Espina V;Petricoin EF 3rd;Liotta LA
通讯作者：
Liotta LA
影响因子：
45.3
作者：
Balch, Charles M.;Gershenwald, Jeffrey E.;Sondak, Vernon K.
通讯作者：
Sondak, Vernon K.