Immunolocalization of Fibronectin during Reparative Dentinogenesis in Human Teeth after Pulp Capping with Calcium Hydroxide
Immunolocalization of Fibronectin during Reparative Dentinogenesis in Human Teeth after Pulp Capping with Calcium Hydroxide
复制标题
氢氧化钙盖髓后人牙修复性牙本质发生过程中纤连蛋白的免疫定位
DOI：
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发表时间：
1996
期刊：
影响因子：
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通讯作者：
H. Ozawa
中科院分区：
文献类型：
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作者：
K. Yoshiba;N. Yoshiba;Hiroaki Nakamura;Masaaki Iwaku;H. Ozawa
已知暴露的牙髓具有形成硬组织屏障（牙本质桥）的能力。牙髓细胞在此过程中分化为成牙本质细胞的确切机制尚不清楚。纤维连接蛋白在牙齿发育过程中对成牙本质细胞的分化起着至关重要的作用。本研究验证了纤维连接蛋白参与替代性成牙本质细胞分化和修复性牙本质形成的初始阶段的假设。我们观察了氢氧化钙[Ca（OH）2]盖髓后，牙本质桥形成过程中Ca（OH）2的免疫组织化学定位。加盖后一天，在TEM水平上观察到晶体结构的沉淀，并在浅表坏死区和下面的牙髓组织之间的界面处观察到细胞碎片。术后7 ~ 10天，这层营养不良性钙化显示纤维连接蛋白阳性反应，牙髓细胞似乎与这层密切相关。在第14天，观察到排列的细胞，其中一些是细长的和成牙本质细胞样的，邻近的adheroriectin阳性的不规则基质。细胞间可见螺旋状荧光。第28天，不规则的纤维基质形成后，形成管状的牙本质样基质，衬有成牙本质样细胞。因此，这似乎是与最初形成的钙化层相关的纤维连接蛋白可能在修复性牙本质形成过程中，牙髓细胞分化为成牙本质细胞中发挥介导作用。
Exposed dental pulp is known to possess the ability to form a hard-tissue barrier (dentin bridge). The exact mechanisms by which pulp cells differentiate into odontoblasts in this process are unknown. Fibronectin has been demonstrated to play a crucial role in odontoblast differentiation during tooth development. This study tested the hypothesis that fibronectin is involved in the initial stages of replacement odontoblast differentiation and reparative dentin formation. We observed its immunohistochemical localization during dentin bridge formation in human teeth, after pulp was capped with calcium hydroxide [Ca(OH)2]. One day after the capping, precipitation of crystalline structures was observed at the TEM level in association with cell debris at the interface between the superficial necrotic zone and underlying pulp tissue. This layer of dystrophic calcification showed positive reaction for fibronectin, and pulp cells appeared to be closely associated with this layer, seven to ten days postoperatively. At 14 days, an alignment of cells, some of which were elongated and odontoblast-like, was observed adjacent to the fibroriectin-positive irregular matrix. Between the cells, corkscrew fiber-like fluorescence was visible. At 28 days, the irregular fibrous matrix was followed by the formation of tubular dentin-like matrix lined with odontoblast-like cells. Therefore, it would seem that fibronectin associated with the initially formed calcified layer might play a mediating role in the differentiation of pulp cells into odontoblasts during reparative dentinogenesis, after pulp was capped with Ca(OH)2.
影响因子：
3
作者：
FITZGERALD, M;CHIEGO, DJ;HEYS, DR
通讯作者：
HEYS, DR