The cysteine-rich protein gene family of Giardia lamblia: loss of the CRP170 gene in an antigenic variant.
The cysteine-rich protein gene family of Giardia lamblia: loss of the CRP170 gene in an antigenic variant.
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贾第鞭毛虫富含半胱氨酸的蛋白质基因家族：抗原变异体中 CRP170 基因的丢失。
DOI：
10.1128/mcb.12.3.1194-1201.1992
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发表时间：
1992
影响因子：
5.3
通讯作者：
Nash,TE
中科院分区：
文献类型：
--
作者：
Adam,RD;Yang,YM;Nash,TE
贾第鞭毛虫在体内和体外表现出丰富的半胱氨酸表面抗原的可变表达。估计库的大小在20到184之间，并且在大约12代的WB分离物体外生长后可以检测到特定变异。在早期的研究中，我们克隆了一种170 kda表面抗原（CRP170）的一部分基因，并通过DNA测序证明它富含半胱氨酸（12%），含有2.6个串联重复的195 bp对序列。该克隆在G.lambliaDNA的Southern印迹上杂交到多个条带，其模式在克隆系之间是可变的，但与CRP170的表达无关。目前，我们已经从WBA6克隆分离物中克隆出了CRP170的近全长cDNA和基因组克隆。此外，我们还从WB1269细胞系（表达CRP72）中分离出一个cDNA克隆，这是一个源自WBA6的抗原变体。序列分析显示，crp170和crp72基因的c端氨基酸同源性显著，表明其具有保守的功能作用，如膜锚定。crp170重复寡核苷酸在hindiii酶切的WBA6 DNA上杂交成约6 kb大小的阶梯状条带，代表了crp170的表达拷贝。相比之下，WB1269中没有与相似大小的片段杂交，这表明WB1269滋养体丢失了crp170基因的表达拷贝。
Giardia lambliatrophozoites demonstrate variable expression of a repertoire of cysteine-rich surface antigens in vitro and in vivo. The size of the repertoire has been estimated at 20 to 184, and specific variants can be detected after approximately 12 generations of in vitro growth for the WB isolate. In earlier studies, we cloned a portion of the gene for a 170-kDa surface antigen (CRP170) and demonstrated by DNA sequencing that it was cysteine rich (12%) and contained 2.6 copies of a tandemly repeated 195-bp pair sequence. The clone hybridized to multiple bands on a Southern blot of G.lambliaDNA in a pattern that was variable among the cloned lines but did not correlate with expression of CRP170. We have now cloned a nearly full length cDNA as well as genomic clones for CRP170 from the WBA6 cloned isolate. In addition, we have isolated a cDNA clone from the WB1269 line (expressing CRP72), an antigenic variant which was derived from WBA6. Sequence analysis of theCRP170andCRP72genes revealed marked C-terminal amino acid homology, suggesting a conserved functional role such as membrane anchoring. TheCRP170repeat oligonucleotide hybridized to a stairstep of bands approximately 6 kb in size onHindIII-digested WBA6 DNA representing the expressed copy(ies) ofCRP170.In contrast, there was no hybridization to a fragment of similar size in WB1269, suggesting that WB1269 trophozoites have lost the expressed copy of theCRP170gene.
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影响因子：
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作者：
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影响因子：
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影响因子：
5.3
作者：
J. Preer
通讯作者：
J. Preer
影响因子：
2.1
作者：
NASH, TE;BANKS, SM;CONRAD, JT
通讯作者：
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贾第鞭毛虫中异常短的微管蛋白 mRNA 前导序列的证据和微管蛋白基因的特征。
DOI：
--
发表时间：
1989
期刊：
Molecular and biochemical parasitology (Print)
影响因子：
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作者：
K. E. Kirk;M. Turner;P. Chakraborty
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P. Chakraborty