Skin bacterial microbiome of a generalist Puerto Rican frog varies along elevation and land use gradients.
Skin bacterial microbiome of a generalist Puerto Rican frog varies along elevation and land use gradients.
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作者：
Hughey MC;Pena JA;Reyes R;Medina D;Belden LK;Burrowes PA
宿主相关微生物群落在动物中普遍存在，具有重要的功能。例如，两栖动物的细菌皮肤微生物群可以在预防或减少两栖壶菌（Batrachochytrium dendroatidis）感染方面发挥作用。有证据表明，环境细菌可能至少是两栖动物皮肤细菌群落的一些成员的来源池，强调了局部环境变化可能破坏微生物群落来源池，这可能对宿主生物的健康至关重要。然而，很少有研究评估两栖动物皮肤微生物组在明确的环境梯度上的变化，因此我们对当地环境条件如何影响微生物组多样性知之甚少。在波多黎各东部（0 ~ 875 m）沿海拔梯度取样了Coqui青蛙，Eleutherodactylus Coqui （N = 77）的皮肤细菌群落，样带分为两种土地利用类型：完整森林（N = 4个点）和干扰森林（N = 3个点）。我们发现α多样性（通过Shannon、Simpson和系统发育多样性指数评估）在不同的地点存在差异，但这种差异与海拔或土地利用无关。另一方面，β多样性（群落结构）随着地点、海拔和土地利用的不同而变化，主要是由于这些群落中某些细菌OTUs（~物种）的相对丰度的变化。重要的是，尽管微生物群多样性不同，但coqui在所有站点中保持了共同的核心微生物群。因此，我们的研究结果表明，环境条件可以影响陆生两栖动物皮肤微生物组的组成，但尽管环境变化，微生物组的某些方面仍然保持一致。
Host-associated microbial communities are ubiquitous among animals, and serve important functions. For example, the bacterial skin microbiome of amphibians can play a role in preventing or reducing infection by the amphibian chytrid fungus, Batrachochytrium dendrobatidis. Evidence suggests that environmental bacteria likely serve as a source pool for at least some of the members of the amphibian skin bacterial community, underscoring the potential for local environmental changes to disrupt microbial community source pools that could be critical to the health of host organisms. However, few studies have assessed variation in the amphibian skin microbiome along clear environmental gradients, and so we know relatively little about how local environmental conditions influence microbiome diversity. We sampled the skin bacterial communities of Coqui frogs, Eleutherodactylus coqui (N = 77), along an elevational gradient in eastern Puerto Rico (0–875 m), with transects in two land use types: intact forest (N = 4 sites) and disturbed (N = 3 sites) forest. We found that alpha diversity (as assessed by Shannon, Simpson, and Phylogenetic Diversity indices) varied across sites, but this variation was not correlated with elevation or land use. Beta diversity (community structure), on the other hand, varied with site, elevation and land use, primarily due to changes in the relative abundance of certain bacterial OTUs (∼species) within these communities. Importantly, although microbiome diversity varied, E. coqui maintained a common core microbiota across all sites. Thus, our findings suggest that environmental conditions can influence the composition of the skin microbiome of terrestrial amphibians, but that some aspects of the microbiome remain consistent despite environmental variation.
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影响因子：
4.2
作者：
Chang CW;Huang BH;Lin SM;Huang CL;Liao PC
通讯作者：
Liao PC
影响因子：
4.6
作者：
Barelli C;Albanese D;Donati C;Pindo M;Dallago C;Rovero F;Cavalieri D;Tuohy KM;Hauffe HC;De Filippo C
通讯作者：
De Filippo C
DOI：
10.1038/ismej.2012.8
发表时间：
2012-08
期刊：
The ISME journal
影响因子：
--
作者：
通讯作者：
--
影响因子：
2.2
作者：
Fournier, David A.;Skaug, Hans J.;Sibert, John
通讯作者：
Sibert, John
DOI：
10.1073/pnas.1014497108
发表时间：
2011-06-14
影响因子：
11.1
作者：
Becker, C. Guilherme;Zamudio, Kelly R.
通讯作者：
Zamudio, Kelly R.