Characterization of Mce4A protein of Mycobacterium tuberculosis: role in invasion and survival.
Characterization of Mce4A protein of Mycobacterium tuberculosis: role in invasion and survival.
复制标题
DOI：
10.1186/1471-2180-8-200
复制
发表时间：
2008-11-19
期刊：
影响因子：
4.2
通讯作者：
Bose M
中科院分区：
文献类型：
--
作者：
Saini NK;Sharma M;Chandolia A;Pasricha R;Brahmachari V;Bose M
mce4操纵子是结核分枝杆菌哺乳动物细胞进入（mce）操纵子的四个同源物之一。该操纵子中的mce4A（Rv3499c）基因与mce1A（Rv0169）同源，其在M.结核我们早期的报道表明，mce4操纵子在培养的芽孢杆菌生长的稳定期和哺乳动物宿主的感染过程中表达。M.携带MCE 4操纵子突变的结核病在感染小鼠中显示生长缺陷和存活率降低。然而，Mce4A蛋白的细胞内定位及其在芽孢杆菌的细胞进入或存活中的直接作用迄今尚未得到证实。通过透射电镜观察，我们证实了重组Mce4A蛋白促进了非致病性大肠杆菌的侵袭。大肠杆菌进入非吞噬性HeLa细胞。我们观察到mce4A基因在重组E.大肠杆菌的感染。使用针对Mce4A蛋白的抗体，我们表明该蛋白定位于M的细胞壁部分。结核病H37Rv静止期培养。Mce4A蛋白在肉汤培养的稳定期表达，并定位于M.结核Mce4A蛋白在非致病性E.大肠杆菌使其能够进入并在HeLa细胞和巨噬细胞内存活。由于Mce4A蛋白在分枝杆菌生长的后期表达，我们的研究结果提高了它在维持持续性结核感染中发挥作用的可能性。
The mce4 operon is one of the four homologues of mammalian cell entry (mce) operons of Mycobacterium tuberculosis. The mce4A (Rv3499c) gene within this operon is homologous to mce1A (Rv0169), that has a role in host cell invasion by M. tuberculosis. Our earlier reports show that mce4 operon is expressed during the stationary phase of growth of the bacillus in culture and during the course of infection in mammalian hosts. M. tuberculosis carrying mutation in mce4 operon shows growth defect and reduced survival in infected mice. However, the intracellular localization of Mce4A protein and its direct role in cell entry or survival of the bacillus has not been demonstrated so far. By transmission electron microscopy we have demonstrated that recombinant Mce4A protein facilitates the invasion of non-pathogenic strain of E. coli into non-phagocytic HeLa cells. We observe that mce4A gene has a role comparable to mce1A in the survival of recombinant E. coli in human macrophages. Using antibodies raised against Mce4A protein, we show that the protein is localized in the cell wall fraction of M. tuberculosis H37Rv stationary phase culture only. Mce4A protein is expressed during the stationary phase of broth culture and localizes in the cell wall fraction of M. tuberculosis. Mce4A protein expressed in non-pathogenic E. coli enables it to enter and survive within HeLa cells and the macrophages. As Mce4A protein is expressed during later phase of mycobacterial growth, our results raise the possibility of it playing a role in maintenance of persistent tubercular infection.
登录
查看更多内容
影响因子：
--
作者：
Kumar, A;Chandolia, A;Bose, M
通讯作者：
Bose, M
影响因子：
5.5
作者：
Aguilar, LD;Infante, E;Pando, RH
通讯作者：
Pando, RH
影响因子：
56.9
作者：
ARRUDA, S;BOMFIM, G;RILEY, LW
通讯作者：
RILEY, LW
DOI：
10.1073/pnas.94.10.5243
发表时间：
1997-05-13
影响因子：
11.1
作者：
MacMicking, JD;North, RJ;Nathan, CF
通讯作者：
Nathan, CF
影响因子：
3.1
作者：
Bermudez, LE;Goodman, J
通讯作者：
Goodman, J