Factors impairing cell proliferation in the granulation tissue of pressure ulcers: Impact of bacterial burden
Factors impairing cell proliferation in the granulation tissue of pressure ulcers: Impact of bacterial burden
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损害压疮肉芽组织细胞增殖的因素：细菌负荷的影响
DOI：
10.1111/wrr.12675
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发表时间：
2018
影响因子：
2.9
通讯作者：
Ichioka S
中科院分区：
文献类型：
--
作者：
Sato T;Abe T;Ichioka S
作者旨在使用细胞增殖标记物Ki-67的免疫组织化学评估损害压疮肉芽组织中细胞增殖的因素。这是一项单中心、横断面研究。该研究包括86例III期或IV期压疮患者。从86例患者中获得两个肉芽组织活检标本。标本用于组织学检查、Ki-67免疫组织化学和细菌计数评估。Ki-67染色细胞的%被视为Ki-67指数。Pearson积差相关系数（r）用于评估Ki-67指数与其他定量变量之间的关系，包括年龄、体重指数、细菌计数（Log 10 CFU/g）、血清白蛋白水平、血红蛋白水平、白色血细胞计数和C-反应蛋白水平。根据性别、糖尿病、吸烟状况和伤口培养，使用Mann-WhitneyU检验比较平均Ki-67指数。使用单变量和多变量线性回归分析评估Ki-67指数与其他参数之间的相关性。Mann-WhitneyU检验显示细菌阳性组的Ki-67指数较低（p= 0.045）。细菌计数与Ki-67指数呈显著负相关（r=-0.325，p = 0.002）。多变量线性回归分析显示，细菌计数是Ki-67指数的重要预测因子。调整后的β系数为−1.34（95%置信区间，−2.01至− 0.66，p < 0.001）。在分离的细菌中，棒状杆菌属和金黄色葡萄球菌与低Ki-67指数显著相关，但铜绿假单胞菌与低Ki-67指数无关。这些结果表明，如Ki-67指数所示，压疮肉芽组织中的细菌计数和细胞增殖之间呈负相关。如果适当处理高细菌负荷，压疮中肉芽组织的形成可能会加速。
The authors aimed to assess the factors that impair cell proliferation in the granulation tissue of pressure ulcers using immunohistochemistry for the cell proliferation marker Ki‐67. This was a single center, cross‐sectional study. The study included 86 patients with stage III or IV pressure ulcers. Two granulation tissue biopsy specimens were obtained from 86 patients. The specimens were used for histological examination, Ki‐67 immunohistochemistry, and bacterial count assessment. The % of Ki‐67‐stained cells was considered as the Ki‐67 index. Pearson's product–moment correlation coefficient (r) was used to assess the relationship between the Ki‐67 index and other quantitative variables, including age, body mass index, bacterial count (Log10 CFU/g), serum albumin level, hemoglobin level, white blood cell count, and C‐reactive protein level. The Mann–WhitneyUtest was used to compare the mean Ki‐67 index according to gender, diabetes, smoking status, and wound culture. Univariate and multivariate linear regression analyses were used to assess the association between the Ki‐67 index and other parameters. The Mann–WhitneyUtest revealed that the bacteria‐positive group had a lower Ki‐67 index (p= 0.045). Bacterial count demonstrated a significant negative correlation with the Ki‐67 index (r= −0.325,p= 0.002). Multivariate linear regression analysis showed that bacterial count was a significant predictor of the Ki‐67 index. The adjusted β‐coefficient was −1.34 (95% confidence interval, −2.01 to −0.66,p< 0.001). Among the isolated bacteria,Corynebacterium spp.andStaphylococcus aureuswere significantly associated with a low Ki‐67 index, butPseudomonas aeruginosawas not. These results suggest a negative relationship between bacterial count and cell proliferation in pressure ulcer granulation tissue, as indicated by the Ki‐67 index. Granulation tissue formation in pressure ulcers may be accelerated if high bacterial load is treated appropriately.
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影响因子：
37.8
作者：
Fleisch, M;Billinger, M;Seiler, C
通讯作者：
Seiler, C
影响因子：
9.4
作者：
BUCHANAN, K;HEIMBACH, DM;COYLE, MB
通讯作者：
COYLE, MB
定期清创是大多数慢性伤口保持健康创面床的主要工具。
影响因子：
1.9
作者：
R. Wolcott;J. P. Kennedy;S. Dowd
通讯作者：
S. Dowd
定量细菌学和延迟伤口闭合。
DOI：
--
发表时间：
1968
期刊：
Surgical forum
影响因子：
--
作者：
M. Robson;C. Lea;J. B. Dalton;J. Heggers
通讯作者：
J. Heggers
细菌生长和皮肤移植存活
DOI：
--
发表时间：
1967
期刊：
影响因子：
--
作者：
T. Krizek;M. Robson;E. Sia;T. Krizek
通讯作者：
T. Krizek