Hypoallergenic Wheat Line (1BS-18H) Lacking ω5-Gliadin Induces Oral Tolerance to Wheat Gluten Proteins in a Rat Model of Wheat Allergy.
Hypoallergenic Wheat Line (1BS-18H) Lacking ω5-Gliadin Induces Oral Tolerance to Wheat Gluten Proteins in a Rat Model of Wheat Allergy.
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缺乏ω5-醇溶蛋白的低变应原性小麦品系（1BS-18 H）在小麦过敏的大鼠模型中诱导对小麦面筋蛋白的口服耐受性。
DOI：
10.3390/foods11152181
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发表时间：
2022-07-22
期刊：
影响因子：
--
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中科院分区：
文献类型：
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作者：
早期摄入食物可以预防与诱导口服耐受（OT）相关的食物过敏的发生。我们开发了Hokushin小麦品系作为缺乏ω5-麦醇溶蛋白的低变应原性小麦（1BS-18 H），ω5-麦醇溶蛋白是小麦依赖性运动诱导过敏反应（WDEIA）的主要变应原。1BS-18 H小麦对ω5-醇溶蛋白的敏感性低于北心小麦。在这里，我们使用小麦过敏的大鼠模型评估了通过早期连续摄入1BS-18 H谷蛋白对谷蛋白和ω5-麦醇溶蛋白的OT诱导。每日口服谷蛋白后，用市售谷蛋白或天然ω5-麦醇溶蛋白皮下免疫大鼠。在免疫前每天口服1BS-18 H谷蛋白5天，可将免疫诱导的谷蛋白或ω5-麦醇溶蛋白特异性IgE和IgG 1抗体的增加抑制至与北神谷蛋白相似的水平。静脉注射谷蛋白或ω5-麦醇溶蛋白不能降低由1BS-18 H或Hokushin谷蛋白诱导的OT大鼠的直肠温度，尽管非OT大鼠的直肠温度降低。总之，在致敏之前的早期连续摄入1BS-18 H小麦诱导了对谷蛋白和ω5-醇溶蛋白的OT。这些发现支持了1BS-18 H小麦通过连续摄入预防小麦过敏（包括WDEIA）的益处。
The early ingestion of food can prevent the onset of food allergy related to inducing oral tolerance (OT). We developed the Hokushin wheat line as a hypoallergenic wheat (1BS-18H) lacking ω5-gliadin, a major allergen of wheat-dependent exercise-induced anaphylaxis (WDEIA). The 1BS-18H wheat had lower ability of sensitization for ω5-gliadin compared with Hokushin wheat. Here, we evaluated the induction of OT to gluten and ω5-gliadin by the early consecutive ingestion of 1BS-18H gluten using a rat model of wheat allergy. Rats were subcutaneously immunized with commercial gluten or native ω5-gliadin following the daily oral administration of gluten. The daily oral administration of 1BS-18H gluten for 5 days before immunization suppressed the increase in gluten- or ω5-gliadin-specific IgE and IgG1 antibodies induced by immunization to a level similar to Hokushin gluten. Intravenous challenge with gluten or ω5-gliadin did not decrease the rectal temperature in rats with OT induced by 1BS-18H or Hokushin gluten, although it was decreased in non-OT rats. In conclusion, the early consecutive ingestion of 1BS-18H wheat before sensitization induced OT to gluten and ω5-gliadin. These findings support the benefit of 1BS-18H wheat to prevent wheat allergy including WDEIA by consecutive ingestion in humans.
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影响因子：
5
作者：
Ma, Yanrong;Wu, Daying;Guo, Lei;Yao, Youhua;Yao, Xiaohua;Wang, Zhonghua;Wu, Kunlun;Cao, Xinyou;Gao, Xin
通讯作者：
Gao, Xin
影响因子：
5.4
作者：
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通讯作者：
Morita, E
DOI：
10.4161/gmcr.2.1.15884
发表时间：
2011-01-01
期刊：
GM crops
影响因子：
--
作者：
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通讯作者：
Allen, Paul V
影响因子：
14.2
作者：
Aihara, Y;Takahashi, Y;Yokota, S
通讯作者：
Yokota, S
影响因子：
2
作者：
Komoto K;Okamoto S;Hamada M;Obana N;Samori M;Imamura T
通讯作者：
Imamura T