Exploring the pattern of phenotypic and genetic polymorphism in the arsenic hyperaccumulator Pteris vittata L. (Chinese brake fern)
Exploring the pattern of phenotypic and genetic polymorphism in the arsenic hyperaccumulator Pteris vittata L. (Chinese brake fern)
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砷超积累植物 Pteris vittata L.（中国刹车蕨）的表型和遗传多态性模式探讨
DOI：
10.1007/s11104-013-1810-1
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发表时间：
2013-06
期刊：
影响因子：
4.9
通讯作者：
Wen-Sheng Shu
中科院分区：
文献类型：
--
作者：
Bing Yang;Alessio Mengoni;Ye-Ling Huang;Xiong-Lei He;Jin-Tian Li;Bin Liao;Mi Zhou;Wen-Sheng Shu
背景和目的在植物中，金属和类金属的超积累被认为是防御食草和寄生的积极选择性状。然而，对于与超积累表型相关的基因的分子多态性模式却鲜有研究。方法对我国32个砷超积累植物蕨类植物Pteris vittata的遗传多态性进行了研究。对19个位点进行了测序，其中9个位点编码推测与As超积累有关的蛋白质。结果在推测与As积累相关的基因中检测到较高的多态性。在任何研究的基因座上均不存在阳性选择的证据。 Mantel 对遗传距离与地理、土壤砷浓度、易位和生物富集因子距离进行的测试显示种群存在部分地理聚类，但遗传距离与土壤或超富集参数之间没有相关性。结论我们的研究表明，P. vittata 中砷的组成性超富集虽然在种质之间存在差异，但在推定与砷超富集相关的基因的蛋白质编码序列上没有产生可检测的特征。这些数据可能与超积累表型的古老起源一致，并且至少对于蛋白质序列而言，该性状暗示基因选择压力的放松。
Background and AimsIn plants, hyperaccumulation of metals and metalloids is considered to be a positively selected trait for defense against herbivory and parasitism. However, few investigations have been performed on the patterns of molecular polymorphism of genes putatively related to the hyperaccumulation phenotype.MethodsWe explored the genetic polymorphism of 32 accessions across China of the arsenic (As)-hyperaccumulator fern Pteris vittata. Nineteen loci, 9 of which code for proteins putatively involved in As hyperaccumulation, were sequenced.ResultsA relatively high polymorphism was detected in genes putatively related to As accumulation. No evidence for positive selection was present at any of the investigated loci. Mantel’s test performed on genetic distances versus geographical, soil As concentration, translocation and bioaccumulation factor distances showed a partial geographical clustering of populations but no correlation between genetic distances and edaphic or hyperaccumulation parameters.ConclusionsOur study suggest that constitutive As hyperaccumulation in P. vittata, though variable between accessions, did not produce detectable signatures on protein coding sequences of gene putatively related to As hyperaccumulation. These data may be in agreement with an ancient origin of the hyperaccumulation phenotype and, at least for the protein sequence, a relaxation of selective pressure on genes implied in this trait.
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影响因子：
9.4
作者：
Frerot, Helene;Faucon, Michel-Pierre;Saumitou-Laprade, Pierre
通讯作者：
Saumitou-Laprade, Pierre
DOI：
10.1046/j.1469-8137.2003.00664.x
发表时间：
2003-02
期刊：
The New phytologist
影响因子：
--
作者：
A. Mengoni;C. Gonnelli;E. Brocchini;F. Galardi;S. Pucci;R. Gabbrielli;M. Bazzicalupo
通讯作者：
A. Mengoni;C. Gonnelli;E. Brocchini;F. Galardi;S. Pucci;R. Gabbrielli;M. Bazzicalupo
影响因子：
5.7
作者：
Galardi, F.;Corrales, I.;Gonnelli, C.
通讯作者：
Gonnelli, C.
影响因子：
9.4
作者：
Assunçao, AGL;Bookum, WM;Ernst, WHO
通讯作者：
Ernst, WHO
DOI：
10.1016/s0584-8547(98)00159-1
发表时间：
1998-09
期刊：
Spectrochimica Acta Part B: Atomic Spectroscopy
影响因子：
--
作者：
Yin Ming;L. Bing
通讯作者：
Yin Ming;L. Bing