Comparison of odontogenic differentiation of human dental follicle cells and human dental papilla cells.
Comparison of odontogenic differentiation of human dental follicle cells and human dental papilla cells.
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人牙囊细胞和人牙乳头细胞牙源分化的比较
DOI：
10.1371/journal.pone.0062332
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Tian W
中科院分区：
文献类型：
--
作者：
Guo L;Li J;Qiao X;Yu M;Tang W;Wang H;Guo W;Tian W
经典的牙齿发育理论认为，牙乳头细胞（DPC）是成牙本质细胞的前体细胞，负责牙本质的发育。然而，我们以前的研究表明，牙囊细胞（DFC）可以分化为成牙本质细胞。为了进一步了解牙本质发育过程，以及牙本质发育过程中DFCs和DPCs的差异，我们对DFCs和DPCs的牙本质分化进行了体外和体内研究。将DFC和DPC分别与经处理的牙本质基质（TDM）组合，然后将其皮下植入小鼠背部8周。结果表明，12种蛋白质存在显著差异，其中磷酸丝氨酸氨基转移酶1（PSAT 1）、缺氧诱导因子1-α（HIF 1A）亚型2和膜联蛋白A2（ANXA 2）亚型1是差异最显著的蛋白质。这些蛋白质与缺氧环境中骨平衡、血管生成和细胞存活的调节有关。DFC和DPC均表达牙源性、神经源性和牙周源性标记物。组织学检查显示DFC和DPC在体内均形成牙髓-牙本质/牙骨质-牙周膜样组织。因此，在TDM存在下，DFC和DPC具有相似的牙源性分化潜能。然而，葡萄糖和氨基酸代谢信号转导和蛋白质合成的差异，观察两种细胞类型。这项研究扩展了我们对牙齿发育的理解，并为使用替代细胞来源进行牙齿再生提供了直接证据。
Classical tooth development theory suggests that dental papilla cells (DPCs) are the precursor cells of odontoblasts, which are responsible for dentin development. However, our previous studies have indicated that dental follicle cells (DFCs) can differentiate into odontoblasts. To further our understanding of tooth development, and the differences in dentinogenesis between DFCs and DPCs, the odontogenic differentiation of DFCs and DPCs was characterized in vitro and in vivo. DFCs and DPCs were individually combined with treated dentin matrix (TDM) before they were subcutaneously implanted into the dorsum of mice for 8 weeks. Results showed that 12 proteins were significantly differential, and phosphoserine aminotransferase 1 (PSAT1), Isoform 2 of hypoxia-inducible factor 1-alpha (HIF1A) and Isoform 1 of annexin A2 (ANXA2), were the most significantly differential proteins. These proteins are related to regulation of bone balance, angiogenesis and cell survival in an anoxic environment. Both DFCs and DPCs express odontogenic, neurogenic and peridontogenic markers. Histological examination of the harvested grafts showed that both DFCs and DPCs form pulp-dentin/cementum-periodentium-like tissues in vivo. Hence, DFCs and DPCs have similar odontogenic differentiation potential in the presence of TDM. However, differences in glucose and amino acid metabolism signal transduction and protein synthesis were observed for the two cell types. This study expands our understanding on tooth development, and provides direct evidence for the use of alternative cell sources in tooth regeneration.
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影响因子：
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作者：
Feng, Ganjun;Jin, Xiaobing;Hu, Jiang;Ma, Haiyun;Gupte, Melanie J.;Liu, Hao;Ma, Peter X.
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影响因子：
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3.6
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