D-glucosamine promotes transfection efficiency during electroporation.
D-glucosamine promotes transfection efficiency during electroporation.
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DOI：
10.1155/2014/485867
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发表时间：
2014
影响因子：
--
通讯作者：
Hayashi Y
中科院分区：
文献类型：
--
作者：
Igawa K;Ohara N;Kawakubo A;Sugimoto K;Yanagiguchi K;Ikeda T;Yamada S;Hayashi Y
D-氨基葡萄糖是一种在医学和牙科学的各个领域中有用的药物。关于细胞膜的稳定性，据报道，直接应用D-葡糖胺可显著抑制缓激肽诱导的伤害性反应。电穿孔通常用于将外源基因有效地导入组织培养细胞中。在转染载体的实验中使用了含有或不含有D-葡糖胺的电穿孔缓冲液。这是第一项间接观察成骨细胞膜在D-葡糖胺应用的电穿孔中对电应力和基因摄取的稳定性和保护作用的研究（质子海绵假说：在与溶酶体融合之前的内体期间的渗透破裂）。转染效率通过转染的绿色荧光蛋白（GFP）在培养细胞（成骨细胞; NOS-1细胞）中的荧光强度来评价。在用D-葡糖胺补充的缓冲液处理的电穿孔样品中，转染效率在一天后增加超过30%。D-氨基葡萄糖的膜吸收是电应力引起膜应力的主要机制。D-氨基葡萄糖的这种新功能对于开发更有效的转化程序是有用的和有意义的。
D-Glucosamine is a useful medicament in various fields of medicine and dentistry. With respect to stability of the cell membrane, it has been reported that bradykinin-induced nociceptive responses are significantly suppressed by the direct application of D-glucosamine. Electroporation is usually used to effectively introduce foreign genes into tissue culture cells. Buffers for electroporation with or without D-glucosamine are used in experiments of transfection vectors. This is the first study to indirectly observe the stability and protection of the osteoblast membrane against both electric stress and gene uptake (the proton sponge hypothesis: osmotic rupture during endosomes prior to fusion with lysosomes) in electroporation with D-glucosamine application. The transfection efficiency was evaluated as the fluorescence intensity of the transfected green fluorescent protein (GFP) in the cultured cells (osteoblasts; NOS-1 cells). The transfection efficiency increased over 30% in the electroporation samples treated with D-glucosamine-supplemented buffer after one day. The membrane absorption of D-glucosamine is the primary mechanism of membrane stress induced by electric stress. This new function of D-glucosamine is useful and meaningful for developing more effective transformation procedures.
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影响因子：
5.1
作者：
Tang, MX;Szoka, FC
通讯作者：
Szoka, FC
影响因子：
39.3
作者：
Borer, Jeffrey S.;Pouleur, Hubert;Zannad, Faiez
通讯作者：
Zannad, Faiez
影响因子：
2.3
作者：
CROLLE, G;DESTE, E
通讯作者：
DESTE, E
DOI：
10.1073/pnas.031577698
发表时间：
2001-01-30
影响因子：
11.1
作者：
Putnam, D;Gentry, CA;Langer, R
通讯作者：
Langer, R
影响因子：
3.5
作者：
Kaida, Kei;Yamashita, Hiromi;Hayashi, Yoshihiko
通讯作者：
Hayashi, Yoshihiko