Optical spectroscopic studies of light-harvesting by pigment-reconstituted peridinin-chlorophyll-proteins at cryogenic temperatures.
Optical spectroscopic studies of light-harvesting by pigment-reconstituted peridinin-chlorophyll-proteins at cryogenic temperatures.
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DOI：
10.1007/s11120-006-9090-8
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发表时间：
2006-10
影响因子：
3.7
通讯作者：
Frank, Harry A.
中科院分区：
文献类型：
--
作者：
Ilagan, Robielyn P.;Chapp, Timothy W.;Hiller, Roger G.;Sharples, Frank P.;Polivka, Tomas;Frank, Harry A.
关键词：
利用低温稳态光谱方法研究了多甲藻素与叶绿素a（Chl a）、叶绿素B（Chl B）、叶绿素d（Chl d）、3-乙酰基叶绿素a（3-acetyl-Chl a）和细菌叶绿素a（BChl a）复合后的脱辅基蛋白（apoprotein）的光谱特性。在10 K的吸收光谱提供更好的分辨率的光谱带比在室温下看到的，并揭示特定的色素-蛋白质的相互作用负责的叶绿素的Qy带的位置。该研究表明，连接到环I的两个蛋白质结合的叶绿素调节Qy和Soret跃迁能量的官能团。荧光激发光谱被用来计算在室温下的各种复合物的能量转移效率，这些与以前报道的超快，时间分辨的光学光谱动力学数据。结果说明了强大的性质和价值的PCP复杂，它保持了高效率的天线功能，即使在存在的非本地叶绿素物种，作为一种有效的工具，阐明光合捕光的分子细节。
Low temperature, steady-state, optical spectroscopic methods were used to study the spectral features of peridinin-chlorophyll-protein (PCP) complexes in which recombinant apoprotein has been refolded in the presence of peridinin and either chlorophyll a (Chl a), chlorophyll b (Chl b), chlorophyll d (Chl d), 3-acetyl-chlorophyll a (3-acetyl-Chl a) or bacteriochlorophyll a (BChl a). Absorption spectra taken at 10 K provide better resolution of the spectroscopic bands than seen at room temperature and reveal specific pigment–protein interactions responsible for the positions of the Qy bands of the chlorophylls. The study reveals that the functional groups attached to Ring I of the two protein-bound chlorophylls modulate the Qy and Soret transition energies. Fluorescence excitation spectra were used to compute energy transfer efficiencies of the various complexes at room temperature and these were correlated with previously reported ultrafast, time-resolved optical spectroscopic dynamics data. The results illustrate the robust nature and value of the PCP complex, which maintains a high efficiency of antenna function even in the presence of non-native chlorophyll species, as an effective tool for elucidating the molecular details of photosynthetic light-harvesting.
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影响因子：
3.3
作者：
Bautista, JA;Connors, RE;Frank, HA
通讯作者：
Frank, HA
影响因子：
2.9
作者：
Fraser, NJ;Dominy, PJ;Cogdell, RJ
通讯作者：
Cogdell, RJ
影响因子：
--
作者：
GUDOWSKANOWAK, E;NEWTON, MD;FAJER, J
通讯作者：
FAJER, J
影响因子：
3.2
作者：
Frigaard, NU;Maresca, JA;Bryant, DA
通讯作者：
Bryant, DA
影响因子：
3.5
作者：
Croce, R;Remelli, R;Bassi, R
通讯作者：
Bassi, R