The diagnostic value of circulating microRNAs for middle-aged (40-60-year-old) coronary artery disease patients.
The diagnostic value of circulating microRNAs for middle-aged (40-60-year-old) coronary artery disease patients.
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循环microRNA对中年(40-60岁)冠心病患者的诊断价值
DOI：
10.6061/clinics/2015(04)07
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发表时间：
2015-04
期刊：
影响因子：
--
通讯作者：
Peng J
中科院分区：
文献类型：
--
作者：
Sayed AS;Xia K;Li F;Deng X;Salma U;Li T;Deng H;Yang D;Haoyang Z;Yang T;Peng J
循环microRNA已被认为是各种疾病的有前途的生物标志物。本研究旨在探讨循环miR-149、miR-424和miR-765作为非侵入性生物标志物在中年（40-60岁）冠心病诊断中的潜在作用。选择年龄、性别、吸烟、高血压和糖尿病等因素相匹配的稳定型冠心病患者65例（49--57岁）、不稳定型冠心病患者30例（49-58岁）和非冠心病患者32例（49--57岁）。用TRIzol试剂从血浆中分离总RNA。通过定量实时聚合酶链反应测量循环miRNA水平。与非冠状动脉疾病患者（5.30 ± 2.57）相比，稳定性冠状动脉疾病患者（1.18 ± 0.84）和不稳定性冠状动脉疾病患者（1.04 ± 0.65）的循环miR-149水平分别降低了4.49倍和5.09倍（p<0.001）。与非冠状动脉疾病患者（4.35 ± 2.20）相比，稳定性冠状动脉疾病患者（1.18 ± 0.60）和不稳定性冠状动脉疾病患者（0.86 ± 0.54）的循环miR-424水平分别降低了3.6倍和5倍（p<0.001）。相比之下，与非冠状动脉疾病患者（1.53 ± 0.99）相比，稳定性冠状动脉疾病患者（6.09 ± 2.27）和不稳定性冠状动脉疾病患者（8.17 ± 2.77）的循环miR-765水平分别升高了3.98倍和5.33倍（p<0.001）。受试者工作特征曲线分析显示，与非冠状动脉疾病患者相比，稳定型CAD患者中循环miR-149、miR-424和miR-765的曲线下面积分别为0.938、0.919和0.968，不稳定型冠状动脉疾病患者中分别为0.951、0.960和0.977。我们的研究结果表明，循环miR-149，miR-424和miR-765可能是新的，非侵入性的生物标志物，用于诊断中年患者的冠状动脉疾病。然而，在得出可靠的结论之前，需要在大型患者队列中进行未来的前瞻性试验。
Circulating microRNAs have been recognized as promising biomarkers for various diseases. The present study aimed to explore the potential roles of circulating miR-149, miR-424 and miR-765 as non-invasive biomarkers for the diagnosis of coronary artery disease in middle-aged (40–60-year-old) patients. Sixty-five stable coronary artery disease patients (49–57 years old), 30 unstable coronary artery disease patients (49–58 years old), and 32 non-coronary artery disease patients (49–-57 years old) who were matched for age, sex, smoking habits, hypertension and diabetes were enrolled in this study. Total RNA was isolated from plasma with TRIzol reagent. Circulating miRNA levels were measured by quantitative real-time polymerase chain reaction. Circulating miR-149 levels were decreased 4.49-fold in stable coronary artery disease patients (1.18 ± 0.84) and 5.09-fold in unstable coronary artery disease patients (1.04 ± 0.65) compared with non-coronary artery disease patients (5.30 ± 2.57) (p<0.001). Circulating miR-424 levels were reduced 3.6-fold in stable coronary artery disease patients (1.18 ± 0.60) and 5-fold in unstable coronary artery disease patients (0.86 ± 0.54) compared with non-coronary artery disease patients (4.35 ± 2.20) (p<0.001). In contrast, circulating miR-765 levels were elevated 3.98-fold in stable coronary artery disease patients (6.09 ± 2.27) and 5.33-fold in unstable coronary artery disease patients (8.17 ± 2.77) compared with non-coronary artery disease patients (1.53 ± 0.99) (p<0.001). Receiver operating characteristic curve analysis revealed that the respective areas under the curve for circulating miR-149, miR-424 and miR-765 were 0.938, 0.919 and 0.968 in stable CAD patients and 0.951, 0.960 and 0.977 in unstable coronary artery disease patients compared with non-coronary artery disease patients. Our results suggest that circulating miR-149, miR-424 and miR-765 might be novel, non-invasive biomarkers for the diagnosis of coronary artery disease in middle-aged patients. However, future prospective trials in large patient cohorts are necessary before reaching a solid conclusion.
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影响因子：
18.2
作者：
Goren, Yaron;Kushnir, Michal;Amir, Offer
通讯作者：
Amir, Offer
影响因子：
37.8
作者：
Go AS;Mozaffarian D;Roger VL;Benjamin EJ;Berry JD;Blaha MJ;Dai S;Ford ES;Fox CS;Franco S;Fullerton HJ;Gillespie C;Hailpern SM;Heit JA;Howard VJ;Huffman MD;Judd SE;Kissela BM;Kittner SJ;Lackland DT;Lichtman JH;Lisabeth LD;Mackey RH;Magid DJ;Marcus GM;Marelli A;Matchar DB;McGuire DK;Mohler ER 3rd;Moy CS;Mussolino ME;Neumar RW;Nichol G;Pandey DK;Paynter NP;Reeves MJ;Sorlie PD;Stein J;Towfighi A;Turan TN;Virani SS;Wong ND;Woo D;Turner MB;American Heart Association Statistics Committee and Stroke Statistics Subcommittee
通讯作者：
American Heart Association Statistics Committee and Stroke Statistics Subcommittee
DOI：
10.1161/atvbaha.114.303425
发表时间：
2014-10
期刊：
Arteriosclerosis, thrombosis, and vascular biology
影响因子：
--
作者：
Kumar S;Kim CW;Simmons RD;Jo H
通讯作者：
Jo H
影响因子：
24
作者：
Fihn, Stephan D.;Gardin, Julius M.;Yancy, Clyde W.
通讯作者：
Yancy, Clyde W.
影响因子：
18.2
作者：
Ellis, Katrina L.;Cameron, Vicky A.;Richards, A. Mark
通讯作者：
Richards, A. Mark