CCCP-Induced LC3 lipidation depends on Atg9 whereas FIP200/Atg13 and Beclin 1/Atg14 are dispensable.
CCCP-Induced LC3 lipidation depends on Atg9 whereas FIP200/Atg13 and Beclin 1/Atg14 are dispensable.
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DOI：
10.1016/j.bbrc.2013.02.010
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发表时间：
2013-03-08
影响因子：
3.1
通讯作者：
Yin, Xiao-Ming
中科院分区：
文献类型：
--
作者：
Chen, Daohong;Chen, Xi;Li, Min;Zhang, Hao;Ding, Wen-Xing;Yin, Xiao-Ming
Treatment of cells with carbonyl cyanide m-chlorophenylhydrazone (CCCP), a mitochondrial proton gradient uncoupler, can result in mitochondrial damage and autophagy activation, which in turn eliminates the injured mitochondria in a Parkin-dependent way. How CCCP mobilizes the autophagy machinery is not fully understood. By analyzing a key autophagy step, LC3 lipidation, we examined the roles of two kinase complexes typically involved in the initiation and nucleation phases of autophagy, namely the ULK kinase complex (UKC) and the Beclin 1/Atg14 complex. We found that CCCP-induced LC3 lipidation could be independent of Beclin 1 and Atg14. In addition, deletion or knockdown of the UKC component FIP200 or Atg13 only led to a partial reduction in LC3 lipidation, indicating that UKC could be also dispensable for this step during CCCP treatment. In contrast, Atg9, which is important for transporting vesicles to early autophagosomal structure, was required for CCCP-induced LC3 lipidation. Taken together, these data suggest that CCCP-induced autophagy and mitophagy depends more critically on Atg9 vesicles than on UKC and Beclin 1/Atg14 complex.
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影响因子：
13.3
作者：
Chu, Charleen T.;Zhu, Jianhui;Dagda, Ruben
通讯作者：
Dagda, Ruben
影响因子：
4.8
作者：
Ding, Wen-Xing;Ni, Hong-Min;Yin, Xiao-Ming
通讯作者：
Yin, Xiao-Ming
DOI：
10.1083/jcb.201202061
发表时间：
2012-07-23
期刊：
The Journal of cell biology
影响因子：
--
作者：
Yamamoto H;Kakuta S;Watanabe TM;Kitamura A;Sekito T;Kondo-Kakuta C;Ichikawa R;Kinjo M;Ohsumi Y
通讯作者：
Ohsumi Y
DOI：
10.1073/pnas.0911267106
发表时间：
2009-12-08
影响因子：
11.1
作者：
Saitoh, Tatsuya;Fujita, Naonobu;Akira, Shizuo
通讯作者：
Akira, Shizuo
影响因子：
13.3
作者：
Itakura, Eisuke;Mizushima, Noboru
通讯作者：
Mizushima, Noboru