Atg9 vesicles are an important membrane source during early steps of autophagosome formation.
Atg9 vesicles are an important membrane source during early steps of autophagosome formation.
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DOI：
10.1083/jcb.201202061
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发表时间：
2012-07-23
期刊：
影响因子：
--
通讯作者：
Ohsumi Y
中科院分区：
文献类型：
--
作者：
Yamamoto H;Kakuta S;Watanabe TM;Kitamura A;Sekito T;Kondo-Kakuta C;Ichikawa R;Kinjo M;Ohsumi Y
含atg9的囊泡组装成自噬体前结构，最终被纳入自噬体外膜。在自噬过程中，细胞质物质被双膜结构（自噬体）隔离，然后被运送到分解室进行降解。关于自噬的一个最基本的问题涉及自噬体膜的起源。在这项研究中，我们重点研究了Atg9的细胞内动力学，Atg9是酵母中自噬体形成所必需的多跨膜蛋白。我们发现绝大多数Atg9存在于细胞质移动囊泡（称为Atg9囊泡）上，这些囊泡来自高尔基体，参与了Atg23和Atg27的过程。我们还发现，单轮自噬体形成只需要几个Atg9囊泡。在饥饿过程中，几个Atg9小泡单独组装成自噬体前结构，并最终被纳入自噬体外膜。我们的发现提供了细胞质Atg9囊泡与自噬体膜之间的结结性联系，并为自噬体形成早期对Atg9囊泡的需求提供了新的见解。
Atg9-containing vesicles assemble to the preautophagosomal structure and eventually are incorporated into the autophagosomal outer membrane. During the process of autophagy, cytoplasmic materials are sequestered by double-membrane structures, the autophagosomes, and then transported to a lytic compartment to be degraded. One of the most fundamental questions about autophagy involves the origin of the autophagosomal membranes. In this study, we focus on the intracellular dynamics of Atg9, a multispanning membrane protein essential for autophagosome formation in yeast. We found that the vast majority of Atg9 existed on cytoplasmic mobile vesicles (designated Atg9 vesicles) that were derived from the Golgi apparatus in a process involving Atg23 and Atg27. We also found that only a few Atg9 vesicles were required for a single round of autophagosome formation. During starvation, several Atg9 vesicles assembled individually into the preautophagosomal structure, and eventually, they are incorporated into the autophagosomal outer membrane. Our findings provide conclusive linkage between the cytoplasmic Atg9 vesicles and autophagosomal membranes and offer new insight into the requirement for Atg9 vesicles at the early step of autophagosome formation.
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影响因子：
64.8
作者：
Ichimura, Y;Kirisako, T;Ohsumi, Y
通讯作者：
Ohsumi, Y
影响因子：
7.7
作者：
Elsner, M;Hashimoto, H;Weiss, M
通讯作者：
Weiss, M
影响因子：
64.5
作者：
Nakatogawa, Hitoshi;Ichimura, Yoshinobu;Ohsumi, Yoshinori
通讯作者：
Ohsumi, Yoshinori
影响因子：
3.4
作者：
KUSUMI, A;SAKO, Y;YAMAMOTO, M
通讯作者：
YAMAMOTO, M
ATG11将ATG9募集到脑吞噬体结构中对于萌芽酵母的选择性自噬至关重要。
影响因子：
7.8
作者：
He, Congcong;Song, Hui;Yorimitsu, Tomohiro;Monastyrska, Iryna;Yen, Wei-Lien;Legakis, Julie E;Klionsky, Daniel J
通讯作者：
Klionsky, Daniel J