Gene expression in rat supraspinatus tendon recovers from overuse with rest.
Gene expression in rat supraspinatus tendon recovers from overuse with rest.
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DOI：
10.1007/s11999-008-0270-z
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发表时间：
2008-07
影响因子：
4.2
通讯作者：
Soslowsky, Louis J.
中科院分区：
文献类型：
--
作者：
Jelinsky, Scott A.;Lake, Spencer P.;Archambault, Joanne M.;Soslowsky, Louis J.
休息是过度使用损伤的常见治疗方法，但其对基因表达的有效性尚未在受控实验条件下进行系统评估。我们询问由于过度使用而在冈上肌腱中受到调节的基因是否会在休息 2 或 4 周后恢复到正常水平。我们使用了肌腱过度使用的大鼠模型，该模型在组织学、几何形状、基因表达和机械特性方面产生与过度使用损伤一致的可重复变化。动物接受 2 或 4 周的过度使用方案，然后休息 2 或 4 周。微阵列分析用于测量过度使用加休息方案后基因表达的整体变化。大多数动物因过度使用而上调的基因在休息后恢复到接近正常水平。休息期后，肌腱的生化成分与正常相似，只是胶原蛋白含量略低。这些结果表明，在该大鼠模型中，只要休息 2 周，通常就足以从 2 周和 4 周过度使用造成的分子和生化影响中恢复。
Rest is a common treatment for overuse injuries, but its effectiveness on gene expression has not been systematically evaluated under controlled experimental conditions. We asked whether genes regulated in the supraspinatus tendon as a result of overuse would return to normal levels after 2 or 4 weeks of rest. We used a rat model of tendon overuse that generates reproducible changes in the histology, geometry, gene expression, and mechanical properties consistent with an overuse injury. Animals were subjected to the overuse protocol for 2 or 4 weeks followed by either 2 or 4 weeks of rest. Microarray analysis was used to measure global changes in gene expression after the overuse plus rest protocol. Genes upregulated as a result of the overuse returned to near normal levels after rest in most animals. The biochemical composition of the tendon was similar to normal after the imposed rest period, except for slightly lower collagen content. These results suggest as little as 2 weeks of rest is often sufficient to recover from the molecular and biochemical effects of 2 and 4 weeks of overuse in this rat model.
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