The cap-snatching frequency of a plant bunyavirus from nonsense mRNAs is low but is increased by silencing of UPF1 or SMG7.
The cap-snatching frequency of a plant bunyavirus from nonsense mRNAs is low but is increased by silencing of UPF1 or SMG7.
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植物布尼亚病毒从无义 mRNA 中抢夺帽子的频率较低，但通过沉默 UPF1 或 SMG7 会增加频率
DOI：
10.1111/mpp.13179
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发表时间：
2022-04
影响因子：
4.9
通讯作者：
Du Z
中科院分区：
文献类型：
--
作者：
Jin J;She Y;Qiu P;Lin W;Zhang W;Zhang J;Wu Z;Du Z
布尼亚病毒切割宿主细胞的mRNA，在一个被称为夺帽的过程中为其自身的mRNA获得帽结构。布尼亚病毒如何与细胞mRNA监视途径相互作用，例如在夺帽过程中无义介导的衰变（NMD）仍然知之甚少，特别是在植物中。水稻条纹病毒（Rice Stripe Virus，RSV）是一种危害东亚水稻生产的植物本扬病毒。在这里，通过一个新开发的系统，我们可以将定义的mRNA呈递给本氏烟草中的RSV，我们发现RSV在夺帽过程中靶向无义mRNA（nsRNA）的频率远低于其靶向正常mRNA的频率。病毒诱导的UPF 1或SMG 7基因沉默增加了RSV靶向nsRNA的频率，UPF 1或SMG 7各自编码参与NMD早期步骤的蛋白质组分（在rdr 6 RNAi背景下）。巧合的是，在UPF 1或SMG 7沉默的植物中RSV积累增加。这些数据表明，在夺帽期间RSV靶向nsRNA的频率受到NMD的限制。通过限制RSV靶向nsRNA的频率，NMD可能会对RSV的总体夺帽效率施加限制。除了对RSV的夺帽作用有了更深入的了解外，这些发现还指出了NMD在植物-布尼亚病毒相互作用中的新作用。与先前的假设相反，水稻条纹病毒（一种植物本扬病毒）从无义mRNA中夺帽的频率受到宿主细胞无义介导的衰变的限制。
Bunyaviruses cleave host cellular mRNAs to acquire cap structures for their own mRNAs in a process called cap‐snatching. How bunyaviruses interact with cellular mRNA surveillance pathways such as nonsense‐mediated decay (NMD) during cap‐snatching remains poorly understood, especially in plants. Rice stripe virus (RSV) is a plant bunyavirus threatening rice production in East Asia. Here, with a newly developed system allowing us to present defined mRNAs to RSV in Nicotiana benthamiana, we found that the frequency of RSV to target nonsense mRNAs (nsRNAs) during cap‐snatching was much lower than its frequency to target normal mRNAs. The frequency of RSV to target nsRNAs was increased by virus‐induced gene silencing of UPF1 or SMG7, each encoding a protein component involved in early steps of NMD (in an rdr6 RNAi background). Coincidently, RSV accumulation was increased in the UPF1‐ or SMG7‐silenced plants. These data indicated that the frequency of RSV to target nsRNAs during cap‐snatching is restricted by NMD. By restricting the frequency of RSV to target nsRNAs, NMD may impose a constraint to the overall cap‐snatching efficiency of RSV. Besides a deeper understanding for the cap‐snatching of RSV, these findings point to a novel role of NMD in plant–bunyavirus interactions. In contrast to previous assumptions, the cap‐snatching frequency of rice stripe virus, a plant bunyavirus, from nonsense mRNAs is restricted by nonsense‐mediated decay of the host cell.
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影响因子：
10.5
作者：
Hopkins, Kaycie C.;McLane, Laura M.;Cherry, Sara
通讯作者：
Cherry, Sara
DOI：
10.1038/nrm3454
发表时间：
2012-11
期刊：
Nature reviews. Molecular cell biology
影响因子：
--
作者：
通讯作者：
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RNA 质量控制是抑制植物内源基因 RNA 沉默的关键。
影响因子：
27.5
作者：
Liu L;Chen X
通讯作者：
Chen X
拟南芥细胞RNA脱帽动力学的变化影响番茄斑萎病毒的帽抢夺和感染
影响因子：
9.4
作者：
Ma, Xiaofang;Zhou, Yijun;Moffett, Peter
通讯作者：
Moffett, Peter
影响因子：
7.2
作者：
Merai, Zsuzsanna;Benkovics, Anna H.;Silhavy, Daniel
通讯作者：
Silhavy, Daniel